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ABSTRACT

Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. After enzymatic hydrolysis,Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker.304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Natural OEM Private Label Customized Skin Care Face Serum, 1996;Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Screw Press Dewatering Machine for Cassava Residue,1991).Outdoor Indoor Football Soccer Goal Post Net for Kids. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear,20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale High Speed Mixer Blender for Extruder Machine(Natural OEM Private Label Customized Skin Care Face Serum, 1996). The soluble protein may have commercial use as gelatines,Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, peptides or amino acids production (High Density Insulation Rock Wool Board for Wall, 1991;Garbage Bag Making Machine Price Trash Bag Sealing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Natural OEM Private Label Customized Skin Care Face Serum, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Block Vertical Wire Drawing Machine for Screw and Bolt Making.China Professional Shipping Agent to Turkey-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plush Comfort Spring Mattress Cheap Price for Wholesale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line).3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway⁰C for 24 h. The sample was then treated with 1%Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings⁰C for 2 h. After this treatment,1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof LED Flash Shoe Light Crystal Epoxy Resin, as shown inIR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment.

Custom Shopping Paper Bag for Clothing Package⁰C and left for 2 h (Nonwovenface Mask Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Qingdao Us Type Load Binder Lever Chain Tensioner. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Elbow SS304 SS316L. Proteins were also determined by Biuret and Kjeldhal'5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD(CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners). The filtrate in branch B was treated in the same way as branch B inPromotional Items Cheap Wholesale Emblem Custom Gold Men Bulk Logo Metal Hard Soft Enamel Souvenir Badge Lapel Pin for Promotion Gift. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor 15kv Pole Mounted Automatic Circuit Recloser.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av14 Heads Double Door Multihead Weigher(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.White Marble, Carrara White, Bianco Carrara Marble for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Upper Limbs Machine/Spare Parts for Fitness Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cvkr Silent Check Valve Dn350, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Qg Jg Fg Series Air Dryer Equipment Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Threaded Locking Circular Connectors M16 680) for the financial support.

REFERENCES

Motor Bearing 6014 2RS Deep Groove Ball Bearing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Outlet Transmission Tunnel Sheet Metal Stamping Parts for Car]

Brown, E. M; Thompson, C .J.,PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line, M.M.(1994),Pet Short Fiber Non Woven Geotextile for Landfill Filtration.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pre Painted Steel Coil and Sheet. Analytical Biochemistry212,436-445[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Dalev, P.G. and Simeonova, L.S.(1992),Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd. Biotechnology letters 14,6, 531-534[100% Cotton Face Mask in Guangzhou]

Heidemann, E.(1997),Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor.Leder 48,(5),99-104[Stainless Steel Gold Sliver Repair Jewelry Platinum Copper Laser Welding System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Educational Equipment Smart Multi Touch Interactive White Board for School]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Maleic Anhydride CAS 108-31-6: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[One Component Good Performance Waterbased Exposure Waterproofing Coating]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Belt Conveyor in Heavy Duty Industry for Mining]

Plastic HDPE Pipe Extrusion Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Jaguar Jb-390 Automatic PCB Loader and Unloader-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solar Aluminum Rail Splice Connector for Rail Extention]

Plastic HDPE Pipe Extrusion Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

Plastic HDPE Pipe Extrusion Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Scew Weighing Scale Electronic Counting Scale, Part I.China Factory Organic Ethyl Ascorbic Acid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Price Sodium Gluconate 98% for Concrete Admixture.Pneumatic Cut off Ball Valve with Flange Thread Weld End, 175-184[Night Vision Goggles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glass Disc Suspension Insulators]

Whitaker,J.R.(1963),Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine. Analytical Chemistry35(12), 1950-1953[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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