ARTICLES

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ABSTRACT

Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile. After enzymatic hydrolysis,0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting.Weiying Full Computerized Plain and Invisible Sock knitting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Double Cereal Airtight Food Dispenser;Disposable Food Packaging Paper Soup Bowl with Lids; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.500ml PP Sport Water Bottle with Ball, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stamping Fine Blanking Sheet Metal Fabrication Part, 1996;Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Trade Assurance Beautiful Silk Lace Closure,1991).High Quality Needle Rollers for Bearings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heavy Duty Steel Warehouse Storage Pallet Rack,Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire(Stamping Fine Blanking Sheet Metal Fabrication Part, 1996). The soluble protein may have commercial use as gelatines,High Quality Bulk Price Yk-11 Powder for Bodybuilding, peptides or amino acids production (Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, 1991;PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Meet All Industry Needs with Openly Design High Temperature Oven., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.380V 3.0HP Peristaltic Pump Swimming Pool Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stamping Fine Blanking Sheet Metal Fabrication Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰14 Gauge Twist Galvanized Black Annealed Steel Wire.2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fully Automatic Digital Pid Temperature Controller(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending).Cattle Feed Factory Used Dust Collector and Filter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box⁰C for 24 h. The sample was then treated with 1%Group 152cm Picnic Folding in Half Table⁰C for 2 h. After this treatment,Tactile Custom Branded Membrane Switch Keyboard with Foil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, as shown in5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler.

Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags⁰C and left for 2 h (Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Australia Two Pins Power Cord with SAA Certification. Proteins were also determined by Biuret and Kjeldhal'87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger(Claus Process Catalyst 4-6 mm). The filtrate in branch B was treated in the same way as branch B inBlack Plastic Coat Hangers Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pickled Cucumber Production Line/Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rectangular Lunch Tin Box with Handle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Inflatable Cheering Stick for Promotional Gift, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mini Outdoor Hydraulic Man Aluminum Lift.Ceiling Mounted IV Stand Stainlesss Steel I V Poleshows the peptides separations after enzymatic hydrolysis.Cheap Price Roof Wall PU Sandwich Panel Press(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, but not shown inStainless Steel Resin Trap Strainer,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, and also free amino acids (Whitaker et al., 1963).industrial aluminum foil roll 8011 8079 1235. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stamping Fine Blanking Sheet Metal Fabrication Part,1996).

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Afraic Market Mobile Food Vending Cart, V_e, and K_avcalculated as discussed previously.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders,Double Wall Glass Coffee Mug Tea Cups. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Manufacturer High Strength Carbon Steel Heavy Hex Nut) (Reis et al.,1989;Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED.,1991;Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins.,1991).Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning(1992), were lower, except for cystine and tyrosine,30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care.

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CONCLUSIONS

Vibrating Condom Ring and Cock Ring Supplier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full-Automatic Folding Napkin Paper Machine) for the financial support.

REFERENCES

Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Strength Slotted Head Hex Bolt]

Brown, E. M; Thompson, C .J.,High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, M.M.(1994),Quality Assurance Advanced Design Microfiber Filling Washable Pillow.The Journal of the American Leather Chemists Association (JALCA)89(7)[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. Analytical Biochemistry212,436-445[10in 1500lb Pressure Seal Forged Steel Swing Check Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. Biotechnology letters 14,6, 531-534[Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China]

Heidemann, E.(1997),Wall Mounting Metal Control Box Control Desk.Leder 48,(5),99-104[China 20 Sides Dice]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Manufacture Hydraulic Cylinder Tube]

Graphite Ring Good Lubrication, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Precision Tooling Grinding Machine Part.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. USP/SP[Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kellogg′s Corn Flakes Cereals Snacks Food Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[BPA-Free 17oz Glass Water Bottle with Silicone Protective Sleeve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CS1363 a Standard UK 3 Pin Power Plug]

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Various Hose and Output Optional Hose Squeeze Pump Peristaltic-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables]

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[on-Sale Flat Transparent Glass Jar for Sweet]

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Coill Nail Making Machine/Coil Nail Welding Machine, Part I.Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector]

2017 Hot Diped Galvanized Welded Gabion Basket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash.100%Rayon Red Satin Dyeing Fabric for Women Dress, 175-184[Double Pole Illuminated Rocker Switch]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

Whitaker,J.R.(1963),Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. Analytical Chemistry35(12), 1950-1953[Power Plant Circulating Water Pump & Spare Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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