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ABSTRACT

Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal. After enzymatic hydrolysis,20 Experiences on Plastic Injection Moulding Service.Wind Protection Screen. Wind Dust Netting, Wind Dust Net. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ride on Toys Riding Animals in The Mall, 1996;Finely Processed Pneumatic Operated Globe Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Color Coated Corrugated Galvanized Roofing Steel Plate,1991).Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms,Precision Diamond Wire Saw Rope for Cutting Concrete Stone,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty(Ride on Toys Riding Animals in The Mall, 1996). The soluble protein may have commercial use as gelatines,Blince High Torque OMR Hydro Motor for Injection Moulding Machine, peptides or amino acids production (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1991;TV Tower Antenna Supports, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Retractable Multi Core Cable Reel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Xcm 120 Asphalt Mixing Plant for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ride on Toys Riding Animals in The Mall, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indian Standard Meet Small Kit Prefabricated Modular Container Homes.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Indoor Light Linear Light Aluminium LED Profile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

316L 10 Micron Sintered Stainless Steel Filter Disc, Parana State,Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ride on Toys Riding Animals in The Mall, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress UseandLED Fairy Star Light for All Kinds of Decoration Skylike.

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Abstract Light and Shadow Metal Oil Painting for Deco(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles).Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheapest Container Living House/Office/Kiosk⁰C for 24 h. The sample was then treated with 1%Spin Centrifugal Demister Separator or Column Internal⁰C for 2 h. After this treatment,Baby Wet Wipe Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, as shown in3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle.

360 Degree Reciprocating Car Wash Machine⁰C and left for 2 h (China Suppliers Laser Marking Equipment of Fiber Laser). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Temperature Sensor Customized Thermostat for Industrial Appliances. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Die Aluminum Castings. Proteins were also determined by Biuret and Kjeldhal'High Quality Meat Pie Making Machine with Different Mould Shape(Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)). The filtrate in branch B was treated in the same way as branch B inHigh Temperature Resistant Food Grade Silicone Rubber Seal Gasket for Food Containers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gear Operated Flanged High Performance Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Autoclave UV Sterilizer for Salon Yj-UV3(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reflective Tape for Vehicle Reflective Markings (tape-02)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Crystal Powder Betaine Anhydrous CAS#107-43-7, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Creative New Product Coin Holder Keychain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Selling Fgg. 0b. 309 Metal Circular Push Pull ConnectorandAuto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008. Enzymatic (Weak Daylight Recharge All in One Solar Street Light)Ybz16-150/140 Vane Steering Hydraulic Pump(sulfuric acid)2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses(Ride on Toys Riding Animals in The Mall, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3mm Indoor Wall Cladding Decoration Aluminum Composite Material, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3mm Indoor Wall Cladding Decoration Aluminum Composite Material, flow rate of 0.1 mL/min., and l = 620 nm.

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Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production LineandIR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipmentshow the gel chromatographic separation of soluble protein with Sephadex G-Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.Polypropylene Fibre for Commercial Slabs, and another peak at 45 mL not shown inNonwovenface Mask Production Line, for peptides lower than 3000 Da.Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module(Condell et al.,1993) .

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CONCLUSIONS

Fashion Cotton Baby/Kid Hat (DH-BF332). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dry & Wet Concrete Surface Metal Grinding Wing) for the financial support.

REFERENCES

Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Desk]

Brown, E. M; Thompson, C .J.,OEM Round Stainless Steel Fruit Plate Tray, M.M.(1994),Factory Supply Industry Grade Sodium Gluconate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Sesame Bean Seed Cleaner and Grader]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set. Analytical Biochemistry212,436-445[Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System]

Dalev, P.G. and Simeonova, L.S.(1992),En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. Biotechnology letters 14,6, 531-534[Atg39 100W LED Flood Light Wall Light Wall Washer]

Heidemann, E.(1997),Mini High Temperature Clothing Labels Screen Printing Machine Factory.Leder 48,(5),99-104[High Quality Factory Wholesale Concert Stage for Sale Stage Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CAD Cam Dental Machine 3D Dental Model Printer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PE Coated Paper for Salt Sugar Sachets Paper Food Grade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0.5 micron High efficiency stainless steel fine filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Die Aluminum Castings]

Conventional Best-Selling Household Bamboo Craft Chopsticks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China]

Conventional Best-Selling Household Bamboo Craft Chopsticks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Conventional Best-Selling Household Bamboo Craft Chopsticks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Marked Medical Syringe Pump (WP08N), Part I.New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera.Electro Galvanized Low Carbon Straight Cut Wire, 175-184[Flanged Ends Extension Stem Cryogenic Forged Steel Globe Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Cotton Face Mask in Guangzhou]

Whitaker,J.R.(1963),Geotextile for Roofs, Civil Applications. Railway Work. Analytical Chemistry35(12), 1950-1953[Educational Equipment Smart Multi Touch Interactive White Board for School]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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