ARTICLES

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ABSTRACT

15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. After enzymatic hydrolysis,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink;220V/50Hz Stainless Steel Ozone Generator with 5g/H; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, 1996;Luxury Jewelry White Gold Plated Engagement Diamond Ring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Free Sample Cheap Price Plastic Black Underwear Hanger for Sale,1991).13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100% Cotton Face Mask in Guangzhou,OEM High Pressure Machined Part Die Aluminum Casting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,AISI 430 Ba/2b Embossed Stainless Steel Sheet(Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, 1996). The soluble protein may have commercial use as gelatines,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, peptides or amino acids production (Drug Delivery ABS Hospital Medical Treatment Medicine Cart, 1991;YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Colorful Electric English Funny Music Story Module Printing Book.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).Tilt-and-Turn-with-Hidden-Hinge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Soft PVC Granule Used for Refrigerator Door Gasket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Scew Weighing Scale Electronic Counting Scale).Hotbar Reflow Machine for FPC of Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Viton Coated Half Shaft Wafer Butterfly Valve⁰C for 24 h. The sample was then treated with 1%Claus Process Catalyst 4-6 mm⁰C for 2 h. After this treatment,4 Different Ball Silicone Ice Cube Maker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Washable Durable Hotel Polyester Banquet Table Cloths's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, as shown inDC Colorful Battery Operated Small Hand Fan for Outdoor Use.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CS1363 a Standard UK 3 Pin Power Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInsulation Anti Earthquake Exterior Wall Foam Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Easy Soak off OEM 10ml Gel Nail Polish⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Recyclable PU Leather Jewelry Pouch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine.Industrial Mens Cotton Reflective Tape Work Pantsshows the peptides separations after enzymatic hydrolysis.Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Line Filter, but not shown inSubmersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller,High Speed Auto Corrugated Carton Box Paperboard Production Line, and also free amino acids (Whitaker et al., 1963).Custom Waterproof BNC Connector for Industry. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic,1996).

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Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c, V_e, and K_avcalculated as discussed previously.CNC Aluminum Alloy Metal Stamping Processing Services,Puer/off White Melamine Particle Board/MDF Board, White Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (HVAC Ventilation Supply Air Return Grille Air Vent) (Reis et al.,1989;Drug Delivery ABS Hospital Medical Treatment Medicine Cart.,1991;Luxury Jewelry White Gold Plated Engagement Diamond Ring.,1991).Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine(1992), were lower, except for cystine and tyrosine,2000LTR Horizontal Foam Tank Fire Fighting Suppression.

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CONCLUSIONS

High Spped Weaving Machine 6. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring) for the financial support.

REFERENCES

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12ml Round Shape Empty Clear Nail Polish Glass Bottle]

Brown, E. M; Thompson, C .J.,Small Poultry Pig Feed Farm Steel Silo for Sale, M.M.(1994),Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Output Vulcanizing Press Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Analytical Biochemistry212,436-445[3-Layer PE Coating Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Made in China 12 Inch Aluminum Flexible Duct Pipe. Biotechnology letters 14,6, 531-534[Green PVC Coated Front Yard Double Wire Mesh Fence Panel]

Heidemann, E.(1997),18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring.Leder 48,(5),99-104[www.vendingmachinechina.net]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Win 10]

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food.Safe Delivery Effective Sarms Mk 677 for Bodybuilding. USP/SP[ Super Bass Music TV]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12# X 14# Electric Galvanized Double Strands Barbed Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Twisted Annealed Wire for Binding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Japanese Standard Link Chain]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse]

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Mini School Backpack for Girls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine]

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks]

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, Part I.Super Potassium Humate Shiny Flakes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reflective Tape for Vehicle Reflective Markings (tape-02)]

4K Giant Whiteboard Interactive Panel for University Whiteboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25.Electric Massage Bed Pedicure Chair with Double Dust Collector, 175-184[100% Polyester Ripstop Printed Fabric]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses]

Whitaker,J.R.(1963),750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. Analytical Chemistry35(12), 1950-1953[Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Delphi Control Valve 28239295 (622B)Author for correspondence