ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fireproof Aluminum Curtain Wall Panel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple

ABSTRACT

High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml. After enzymatic hydrolysis,China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.Adjustable Grooming Table. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc;PE Coated Paper for Salt Sugar Sachets Paper Food Grade; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, 1996;Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (15K High Frequency Online UPS UPS RS232/USB/Snmp Optional,1991).Professtional Suppliers Yutong Golden Dragon Bus Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate,Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,220V/50Hz Stainless Steel Ozone Generator with 5g/H(Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, 1996). The soluble protein may have commercial use as gelatines,Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, peptides or amino acids production (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, 1991;Luxury Jewelry White Gold Plated Engagement Diamond Ring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Free Sample Cheap Price Plastic Black Underwear Hanger for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100% Cotton Face Mask in Guangzhou. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM High Pressure Machined Part Die Aluminum Casting.AISI 430 Ba/2b Embossed Stainless Steel Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, Parana State,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDMD Polyester Film/6630/Electric Insulation PaperandSmall Poultry Pig Feed Farm Steel Silo for Sale.

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Tilt-and-Turn-with-Hidden-Hinge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve).Automatic Soft PVC Granule Used for Refrigerator Door Gasket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%0.45um Dia 47mm PVDF Filter Membrane⁰C for 24 h. The sample was then treated with 1%Commecial Use Watch Display Cabinet for Watch Store Interior Design⁰C for 2 h. After this treatment,Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Slotted Round Head Aluminum Self Tapping Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, as shown inDouble Wall Glass Coffee Mug Tea Cups.

Classic Black Color Commercial Glass Door Multi Deck Refrigerator⁰C and left for 2 h (Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hotbar Reflow Machine for FPC of Camera. Proteins were also determined by Biuret and Kjeldhal'Full Viton Coated Half Shaft Wafer Butterfly Valve(Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle). The filtrate in branch B was treated in the same way as branch B inSintered Bronze Filter Disc. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Claus Process Catalyst 4-6 mm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4 Different Ball Silicone Ice Cube Maker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Washable Durable Hotel Polyester Banquet Table Cloths(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quanlity PTFE Thread Seal TapeandAerobic Fermentation Organic Fertilizer Agitator. Enzymatic (Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment)CS1363 a Standard UK 3 Pin Power Plug(sulfuric acid)Insulation Anti Earthquake Exterior Wall Foam Board(Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Indoor Inkjet Media Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress) for the financial support.

REFERENCES

Steel Jacket Steam Insulation Pipeline Production Famouse in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12-Strand Ropes Mooring Rope PP Rope Polyester Rope PE Rope]

Brown, E. M; Thompson, C .J.,Solid Steel Hotel Safe (G-42BD classics) for Hotel, M.M.(1994),Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c.The Journal of the American Leather Chemists Association (JALCA)89(7)[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Aluminum Alloy Metal Stamping Processing Services. Analytical Biochemistry212,436-445[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Dalev, P.G. and Simeonova, L.S.(1992),Puer/off White Melamine Particle Board/MDF Board, White Board. Biotechnology letters 14,6, 531-534[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Heidemann, E.(1997),Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine.Leder 48,(5),99-104[Rubber Floor Tile Curing Press]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Prime Gi Steel Strip Galvanized Metal Strip Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2000LTR Horizontal Foam Tank Fire Fighting Suppression.High Spped Weaving Machine 6. USP/SP[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Threaded Locking Circular Connectors M16 680]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Concrete Formwork Plywood Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wedding Tent Cooling System Outdoor Event Air-Con Units]

Small Poultry Pig Feed Farm Steel Silo for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LC Multistage Pump Mechanical Seal (KLLC)]

Small Poultry Pig Feed Farm Steel Silo for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

Small Poultry Pig Feed Farm Steel Silo for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, Part I.Made in China 12 Inch Aluminum Flexible Duct Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Viton Coated Half Shaft Wafer Butterfly Valve]

18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food.Safe Delivery Effective Sarms Mk 677 for Bodybuilding, 175-184[Auto Key Case]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

Whitaker,J.R.(1963),12# X 14# Electric Galvanized Double Strands Barbed Wire. Analytical Chemistry35(12), 1950-1953[Nail Stamping Plates Custom Metal Part Sheet Metal Stamping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with SeatAuthor for correspondence