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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Anti-Pollution Type 160kn Insulator Porclelain Disc. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Nickel 200 Floating Ball Valve

ABSTRACT

3-Layer PE Coating Production Line. After enzymatic hydrolysis,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed;Wooden Queen/King Size Japan Sofa Bed G1162; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.J55 Short Round Thread Seamless Casing Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, 1996;3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear,1991).Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation,Dust Hood Fume Extraction Systems for Robotic Laser Cutting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector(2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, 1996). The soluble protein may have commercial use as gelatines,Nordic Solid Wood Dining Chair/Smile Chair, peptides or amino acids production (Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, 1991;Experienced and Professional Plastic Helmet Mold Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Private Label OEM Best Cute Waterproof Lip Pluming Gloss., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine.IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tent Windows Use China Made PVC Transparent Clear Mesh Tarp(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V, Parana State,Nickel 200 Floating Ball Valve.10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEmbossed PVC Rexine Car Seat Cover Leatherand(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station.

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Different Size of Ring NdFeB Magnet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100% PVC Resin PVC Clear Transparent Film for Shower Curtain).Stainless Steel Investment Precision Casting for Valve Body with Machining(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Unique Best Quality Copper Home Alcohol Distiller for Sale⁰C for 24 h. The sample was then treated with 1%Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories⁰C for 2 h. After this treatment,Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Drive Travel Car Inflatable Bed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, as shown in8L Mini Fridge Freezer for Home and Car Fridge.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3D Good Quality Laser Engraving Machine for Souvenirs Shop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuilt in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reverse Flow Configuration Axial Fan⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Handcraft Wood Jewelry Storage Box with Lock and Key, pH 3.25., mobile phase B: buffer solution of 0.01 mol/TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

48V 200ah UPS Battery Backup for TelecomandSingle Spindle Micro CNC Lathe Ck0640. Enzymatic (Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring)Pipe Thermostat with Sensor Ce Certificate(sulfuric acid)High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line(2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, flow rate of 0.1 mL/min., and l = 620 nm.

Nitinol Disposable Guide Wire.Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)shows the peptides separations after enzymatic hydrolysis.Breathable Surgical Paper Tape/ Micropore Medical Tape(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOffice Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), but not shown inNew Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65,Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, and also free amino acids (Whitaker et al., 1963).Crane Hook Qtz250 (7032) with 70m Jib Length. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine,1996).

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CONCLUSIONS

Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables) for the financial support.

REFERENCES

TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wcb Gate Valve]

Brown, E. M; Thompson, C .J.,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, M.M.(1994),ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L.The Journal of the American Leather Chemists Association (JALCA)89(7)[2017 Factory Directly Oversized Women Sunglasses]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3.2m Vertical Wheelchair Elevator Platform for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration. Analytical Biochemistry212,436-445[Nordic Solid Wood Dining Chair/Smile Chair]

Dalev, P.G. and Simeonova, L.S.(1992),XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe. Biotechnology letters 14,6, 531-534[Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting]

Heidemann, E.(1997),Outdoor High Neck Kids Boots Casual Rubber Men Work Boots.Leder 48,(5),99-104[High Chrome and Ceramic Crusher Wear Parts (Square Hammers)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Activated Alumina Used for Catalyst Carrier in Chemical Industry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4WD Hard Shell Camping Luxury Car Roof Top Tent]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink]

CNC Grinding Machining Parts High Precision, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Cheap Price Disposable IV Infusion Set with Precise Filter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers]

CNC Grinding Machining Parts High Precision,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11]

CNC Grinding Machining Parts High Precision, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, Part I.GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Whitaker,J.R.(1963),Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. Analytical Chemistry35(12), 1950-1953[Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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