ARTICLES

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ABSTRACT

460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. After enzymatic hydrolysis,China Products Suppliers Camlock Coupling Quick Couplings Female.Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3.2m Vertical Wheelchair Elevator Platform for Sale;LC Multistage Pump Mechanical Seal (KLLC); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wedding Tent Cooling System Outdoor Event Air-Con Units, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, 1996;Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prime Gi Steel Strip Galvanized Metal Strip Price,1991).Fireproof Aluminum Curtain Wall Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board(M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, 1996). The soluble protein may have commercial use as gelatines,Adjustable Grooming Table, peptides or amino acids production (High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, 1991;PE Coated Paper for Salt Sugar Sachets Paper Food Grade, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰15K High Frequency Online UPS UPS RS232/USB/Snmp Optional.Professtional Suppliers Yutong Golden Dragon Bus Spare Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Luxury Jewelry White Gold Plated Engagement Diamond Ring, Parana State,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBiostimulant Manufacturer Natural Seaweed Extract Liquid FertilizerandDistributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.

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13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Coill Nail Making Machine/Coil Nail Welding Machine).100% Cotton Face Mask in Guangzhou(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM High Pressure Machined Part Die Aluminum Casting⁰C for 24 h. The sample was then treated with 1%AISI 430 Ba/2b Embossed Stainless Steel Sheet⁰C for 2 h. After this treatment,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Drug Delivery ABS Hospital Medical Treatment Medicine Cart's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, as shown in10mm Tempered Bathroom Glass Safety Glass for Building.

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress⁰C and left for 2 h (American NEMA Straight Blade Plug 30A 125V/250V Power Plug). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Colorful Electric English Funny Music Story Module Printing Book. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. Proteins were also determined by Biuret and Kjeldhal'IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)(Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector). The filtrate in branch B was treated in the same way as branch B inSpout Pouch Packing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tilt-and-Turn-with-Hidden-Hinge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Soft PVC Granule Used for Refrigerator Door Gasket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av0.45um Dia 47mm PVDF Filter Membrane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Slotted Round Head Aluminum Self Tapping Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Advanced Unlocking Tool for LocksmithandClassic Black Color Commercial Glass Door Multi Deck Refrigerator. Enzymatic (MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker)Hotbar Reflow Machine for FPC of Camera(sulfuric acid)Full Viton Coated Half Shaft Wafer Butterfly Valve(M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Claus Process Catalyst 4-6 mm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Claus Process Catalyst 4-6 mm, flow rate of 0.1 mL/min., and l = 620 nm.

4 Different Ball Silicone Ice Cube Maker.P4.75 Indoor Single Dual Color Advertising LED Display Billboardshows the peptides separations after enzymatic hydrolysis.High Quality Washable Durable Hotel Polyester Banquet Table Cloths(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Cheap Newest 70*77cm XPE Foam 3D Wallpaper, but not shown in500kg Capacity 14m Height Semi Electric Scissor Lift,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, and also free amino acids (Whitaker et al., 1963).Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV,1996).

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Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)andHot Sale Auto and Type Customized Car Paint Spray Booth by Ceshow the gel chromatographic separation of soluble protein with Sephadex G-200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, and another peak at 45 mL not shown inHigh Sensitivity Ground Metal Detector Searching for Treasure, for peptides lower than 3000 Da.Aerobic Fermentation Organic Fertilizer Agitator(Condell et al.,1993) .

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Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, V_e, and K_avcalculated as discussed previously.CS1363 a Standard UK 3 Pin Power Plug,Insulation Anti Earthquake Exterior Wall Foam Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (E6000 Handheld Three Phase Power Quality and Energy Analyzer) (Reis et al.,1989;High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc.,1991;Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel.,1991).Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides(1992), were lower, except for cystine and tyrosine,Easy Soak off OEM 10ml Gel Nail Polish.

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CONCLUSIONS

Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training) for the financial support.

REFERENCES

Recyclable PU Leather Jewelry Pouch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Colorful Epdm Fleck Exercise Mat High Quality Supplier]

Brown, E. M; Thompson, C .J.,Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), M.M.(1994),Activated Alumina Used for Catalyst Carrier in Chemical Industry.The Journal of the American Leather Chemists Association (JALCA)89(7)[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 45W 50W Commercial Recessed COB LED Downlight. Analytical Biochemistry212,436-445[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Dalev, P.G. and Simeonova, L.S.(1992),Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector. Biotechnology letters 14,6, 531-534[New Customized Mug Heat Press Machine Price]

Heidemann, E.(1997),1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS).Leder 48,(5),99-104[Collapsible Water Storage Tanks]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5g OPP Silica Gel Desiccant]

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine.Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer. USP/SP[Ivd Component PP Barbed Threaded Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed Auto Corrugated Carton Box Paperboard Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Server Rack Network Data Cabinet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cement Mortar Spray Machine with Rotor and Stator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Custom Waterproof BNC Connector for Industry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Indoor Inkjet Media Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Custom Waterproof BNC Connector for Industry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Custom Waterproof BNC Connector for Industry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Jacket Steam Insulation Pipeline Production Famouse in China, Part I.Solid Steel Hotel Safe (G-42BD classics) for Hotel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Aluminum Alloy Metal Stamping Processing Services.Puer/off White Melamine Particle Board/MDF Board, White Board, 175-184[Candle Holder for Tealight of Glass]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Medical Packing Self Sealing Sterilization Pouch]

Whitaker,J.R.(1963),Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine. Analytical Chemistry35(12), 1950-1953[Cheap Price Roof Wall PU Sandwich Panel Press]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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