ARTICLES

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ABSTRACT

Mini Outdoor Hydraulic Man Aluminum Lift. After enzymatic hydrolysis,Cheap Price Roof Wall PU Sandwich Panel Press.Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, 1996;Afraic Market Mobile Food Vending Cart., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders,1991).Double Wall Glass Coffee Mug Tea Cups. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning,30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vibrating Condom Ring and Cock Ring Supplier(Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, 1996). The soluble protein may have commercial use as gelatines,Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, peptides or amino acids production (High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, 1991;Quality Assurance Advanced Design Microfiber Filling Washable Pillow, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wall Mounting Metal Control Box Control Desk. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Precision Tooling Grinding Machine Part.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Kellogg′s Corn Flakes Cereals Snacks Food Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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100%Rayon Red Satin Dyeing Fabric for Women Dress(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display).Promotional Gifts Customized Woven Polyester Lanyard for VIP Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Density Artificial Grass Football Playground Surfaces (STO)⁰C for 24 h. The sample was then treated with 1%High Quality Male Water Quick Release Coupling⁰C for 2 h. After this treatment,Professional Manufacture Hydraulic Cylinder Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chenille Microfiber Mop Head's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)HDPE Auto-Deflashing Plastic Bottle Blowing Machine, as shown inBrown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes.

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable⁰C and left for 2 h (Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Industrial Grade Anhydrous Sodium Sulfate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension. Proteins were also determined by Biuret and Kjeldhal'316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube(Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending). The filtrate in branch B was treated in the same way as branch B inRolls for Rolling Mill. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Cost Prefab Steel Poultry Chicken House on Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Blue Color Fish Paper/6520/6521 Flexible Composites. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBig Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Grip Wood Handle Shovel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, V_e, and K_avcalculated as discussed previously.HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream,Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment) (Reis et al.,1989;High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40.,1991;Afraic Market Mobile Food Vending Cart.,1991).China Hebei Phosphating Black Heading Machine Drywall Screw(1992), were lower, except for cystine and tyrosine,Strong Rare Earth Permanent Wind Driven Generator Magnets.

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CONCLUSIONS

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Coill Nail Making Machine/Coil Nail Welding Machine) for the financial support.

REFERENCES

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cast Iron Dual Plates Double Disc Swing Check Valve]

Brown, E. M; Thompson, C .J.,Industry Fluorine Lined Butterfly Valve Lug Type, M.M.(1994),Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer High Strength Carbon Steel Heavy Hex Nut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti-Pollution Type 160kn Insulator Porclelain Disc]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack. Analytical Biochemistry212,436-445[A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper. Biotechnology letters 14,6, 531-534[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Heidemann, E.(1997),Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden.Leder 48,(5),99-104[Mast Mobile Type Container Crane]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anti-Pollution Type 160kn Insulator Porclelain Disc: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Manufacture Hydraulic Cylinder Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.haizhilimachinery.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm]

Nickel 200 Floating Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3-Layer PE Coating Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)]

Nickel 200 Floating Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale]

Nickel 200 Floating Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, Part I.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CS1363 a Standard UK 3 Pin Power Plug]

Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wooden Queen/King Size Japan Sofa Bed G1162.J55 Short Round Thread Seamless Casing Pipe, 175-184[Lipo Laser Slimming Cavitation Ultrasonic RF Cryolipolysis Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables]

Whitaker,J.R.(1963),2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine. Analytical Chemistry35(12), 1950-1953[on-Sale Flat Transparent Glass Jar for Sweet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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