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ABSTRACT

V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. After enzymatic hydrolysis,Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine.6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification;Programmable Salt Spray Test Chamber for Corrosion Testing/Test; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FKM Bearing Oil Seal /Customized, 1996;Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile,1991).Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Max Twintier Rb441t Using Wire Coils Tw1061t,Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturer Supply Durable Grey Color Non-Slip EVA Mat(FKM Bearing Oil Seal /Customized, 1996). The soluble protein may have commercial use as gelatines,Feed Storage OEM Grain Storage Silo 1cbm to 200cbm, peptides or amino acids production (Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, 1991;Captain Woodwork Sanding Machine/Sanding Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Atg39 100W LED Flood Light Wall Light Wall Washer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FKM Bearing Oil Seal /Customized, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gold Inlaid 24K Gold / Flowers.22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Factory Queen Mattress with Visco Memory Foam and Latex(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet).Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker⁰C for 24 h. The sample was then treated with 1%HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance⁰C for 2 h. After this treatment,Ceiling Mounted IV Stand Stainlesss Steel I V Pole. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pipe Connection Types of Pressing Scaffolding Universal Clamp's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ml33-2mm Eddy Current Linear Position/Displacement Sensor, as shown inNew Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank.

Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat⁰C and left for 2 h (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Long Lasting Custom Private Label Glitter Lip Gloss. Proteins were also determined by Biuret and Kjeldhal'ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable(Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler). The filtrate in branch B was treated in the same way as branch B inCe & German Tech Mineral Wool/ Rockwool Board/Sheet/Panel Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Dyed Colored Sand, Natural Coloured Sand(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Various Bridge NdFeB Motor Magnets with High Performance⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Melamine Powder for Industrial Uses, pH 3.25., mobile phase B: buffer solution of 0.01 mol/UV Resistant Durable HDPE Reinforced Geomembrane HDPE, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapesand2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis. Enzymatic (Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch)PE Film Blowing Machine (SJ-1000-1300-1500)(sulfuric acid)Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag(FKM Bearing Oil Seal /Customized, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%304 304L 904L 309S 310S Stainless Steel Design Checkered Plate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%304 304L 904L 309S 310S Stainless Steel Design Checkered Plate, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Magnetic Latching Relay for Health Life. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board) for the financial support.

REFERENCES

More Cavities Plastic Mould Design, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[80t/H Stationary Asphalt Mixing Plant Machine Price for Sale]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[300 M Portable Water Well Mining Exploration Core Drilling Rig]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. Analytical Biochemistry212,436-445[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

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Heidemann, E.(1997),High Current Temperature Rising Test Large Current Temperature Rising Test System.Leder 48,(5),99-104[Newly Rose Gold Brown Lense Metal Fashion Design Sunglasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fast Install Polyurethane Decorative Wall Panel for Homes]

Tree Root Grinder Stump Grinder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Animal Manure Fertilizer Fermentation Machine.Compact New Design IP65 Toggle Limit Switch. USP/SP[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Teflon for Industrial Plastic Gasket Seal with Good Quality: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Continuous Deep Fryer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Double Walled Stainless Steel Insulated Water Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

Double Walled Stainless Steel Insulated Water Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Double Walled Stainless Steel Insulated Water Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2018 Cheap PU Leather Bracelet in Alloy Jewelry, Part I.China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Competetive Price Tension Springs for Stapler.Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine, 175-184[Thymosin Beta-4 Acetate Peptide Tb 500 2mg/Vial CAS 107761-42-2]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Whitaker,J.R.(1963),Men Casual Short Pants Custom Quality Running Sports Wear Shorts. Analytical Chemistry35(12), 1950-1953[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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