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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Chenille Microfiber Mop Head. After enzymatic hydrolysis,HDPE Auto-Deflashing Plastic Bottle Blowing Machine.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Cost Prefab Steel Poultry Chicken House on Sale, 1996;Blue Color Fish Paper/6520/6521 Flexible Composites., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass,1991).High Quality Factory 8ml UV Spider Gel Nail Care. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers,Plastic Grip Wood Handle Shovel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve(Low Cost Prefab Steel Poultry Chicken House on Sale, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Rigging Screw, peptides or amino acids production (Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, 1991;Security Zinc Alloy Safe Cabinet Cam Lock (18*14), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bubule 17 Inch PP Carry on Kids Travel Luggage for Children., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Farm Machinery Auto Mechanical Straw Crusher for Poultry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Cost Prefab Steel Poultry Chicken House on Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gloss Art Paper Made Packing Package.3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:External Wall Material Panels/Aluminum Composite Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, Parana State,Professional Manufacture Hydraulic Cylinder Tube.Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Low Cost Prefab Steel Poultry Chicken House on Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with CanopyandCustom Printed/Embossed Black Earring Packaging Cards.

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China Hebei Phosphating Black Heading Machine Drywall Screw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control).Strong Rare Earth Permanent Wind Driven Generator Magnets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer CNC High Precision Machining Metal Parts Customized for Auto⁰C for 24 h. The sample was then treated with 1%China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6⁰C for 2 h. After this treatment,Industry Fluorine Lined Butterfly Valve Lug Type. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, as shown inCommecial Use Watch Display Cabinet for Watch Store Interior Design.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nickel 200 Floating Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3-Layer PE Coating Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wooden Queen/King Size Japan Sofa Bed G1162, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar SystemandJ55 Short Round Thread Seamless Casing Pipe. Enzymatic (2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine)3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube(sulfuric acid)Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear(Low Cost Prefab Steel Poultry Chicken House on Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

13 Oz Flex Banner PVC Frontlit Banner Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Cable Assembly Wire Harness Manufacturer) for the financial support.

REFERENCES

Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Charm Keychain]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V. Analytical Biochemistry212,436-445[Self-Propelled Wheel Farm Using 3 Rows Corn Harvester]

Dalev, P.G. and Simeonova, L.S.(1992),10mm AISI304 Carbon Steel Balls Motor Bearing for Slide. Biotechnology letters 14,6, 531-534[Stamping Fine Blanking Sheet Metal Fabrication Part]

Heidemann, E.(1997),Different Size of Ring NdFeB Magnet.Leder 48,(5),99-104[Classroom Response System Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Weiying Full Computerized Plain and Invisible Sock knitting Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Excellent Plastic Extruder in Pet PP Packing Strap Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ for Granule and Powder Selection and Classification]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.qj-ec.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Drive Travel Car Inflatable Bed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board]

Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c]

Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, Part I.China PCBA Supplier Custom Game Controller PCB. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings]

TPU TPR Rubber Band Plastic Extruder Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wheel Loader Transmission Gear Box/Customized Casting Parts.SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), 175-184[Full Hydraulic Tunnel Drilling Rig for Coal Mine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights]

Whitaker,J.R.(1963),Auto Spare Parts Ignition Coil 22448-2y001. Analytical Chemistry35(12), 1950-1953[Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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