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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Truck Air Dryer cartridge 14bar, 1996;Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3mm White Waterproof PVC Foam Board,1991).Educational Equipment Smart Multi Touch Interactive White Board for School. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike,Women Party Jewelry 45cm Sequins Choker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity(Truck Air Dryer cartridge 14bar, 1996). The soluble protein may have commercial use as gelatines,High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, peptides or amino acids production (High Freqency PCB with Blind Via, 1991;Strong Aluminum Armrest Reclining Hydraulic Barber Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory SMD3528 60LEDs/M Flexible LED Strip., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot-Press Small Continuous Diamond Saw Blade Cutting Disc.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Truck Air Dryer cartridge 14bar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick.Comfort Furniture Natural Spring Memory Foam Mattress-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Most Popular Split Pressurized Flat Panel Solar Water Heater(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine).Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine⁰C for 24 h. The sample was then treated with 1%Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag⁰C for 2 h. After this treatment,Customized UV Drying Heating Machine for Screen Printer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, as shown inWomen Summer Swimsuit Chest Zip Bathing Suit.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Products Suppliers Camlock Coupling Quick Couplings Female. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEmsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LC Multistage Pump Mechanical Seal (KLLC)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wedding Tent Cooling System Outdoor Event Air-Con Units, pH 3.25., mobile phase B: buffer solution of 0.01 mol/M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Portable Scooters Folding Electric Scooters for Adults) for the financial support.

REFERENCES

Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Vehicle Sharing Scooter]

Brown, E. M; Thompson, C .J.,Luxury Jewelry White Gold Plated Engagement Diamond Ring, M.M.(1994),Free Sample Cheap Price Plastic Black Underwear Hanger for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay. Analytical Biochemistry212,436-445[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

Dalev, P.G. and Simeonova, L.S.(1992),100% Cotton Face Mask in Guangzhou. Biotechnology letters 14,6, 531-534[Trade Assurance Beautiful Silk Lace Closure]

Heidemann, E.(1997),OEM High Pressure Machined Part Die Aluminum Casting.Leder 48,(5),99-104[CNC Metal Parts Manufacture / Precision CNC Turning Part / CNC Part Price/ Metal Lathe / CNC Router Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Kiet Hydraulic Pipe Bender Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Drug Delivery ABS Hospital Medical Treatment Medicine Cart: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Disposable Sugarcane Pulp Paper Bowl 500ml]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Wholesale Shandong Supplier PPGI Steel Coil for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[industrial aluminum foil roll 8011 8079 1235]

YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box]

YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger]

YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Colorful Electric English Funny Music Story Module Printing Book, Part I.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tilt-and-Turn-with-Hidden-Hinge.Automatic Soft PVC Granule Used for Refrigerator Door Gasket, 175-184[Cat Scratch Furniture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

Whitaker,J.R.(1963),0.45um Dia 47mm PVDF Filter Membrane. Analytical Chemistry35(12), 1950-1953[Tactile Custom Branded Membrane Switch Keyboard with Foil]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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