ARTICLES

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ABSTRACT

8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). After enzymatic hydrolysis,Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion.Maleic Anhydride CAS 108-31-6. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic HDPE Pipe Extrusion Making Machine;Jaguar Jb-390 Automatic PCB Loader and Unloader; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Scew Weighing Scale Electronic Counting Scale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Factory Organic Ethyl Ascorbic Acid, 1996;Aluno Aluminum Veneer Price Aluminium Curtain Wall Details., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Price Sodium Gluconate 98% for Concrete Admixture,1991).Pneumatic Cut off Ball Valve with Flange Thread Weld End. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine,Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor(China Factory Organic Ethyl Ascorbic Acid, 1996). The soluble protein may have commercial use as gelatines,Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, peptides or amino acids production (Steel Grating for Industrial Platform, 1991;50Hz/60Hz Low Frequency Home Power Inverter 5000W, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Factory Organic Ethyl Ascorbic Acid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring.3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lint Free Nail Gel Polish Remover Nonwoven Wipes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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KZ-19/16/13 Pneumatic Combination Steel Binding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (9mm/12mm Seven Color Exposed Luminous Pixel String LED).Medical Packing Self Sealing Sterilization Pouch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Filter Cartridge PP Sediment Filter Cartridge⁰C for 24 h. The sample was then treated with 1%Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale⁰C for 2 h. After this treatment,Disposable Skin Stapler (CSPF-35W). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Customized Mug Heat Press Machine Price, as shown in3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer Garbage Incinerator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4 Heads High Speed Professional Paper Cardboard Laser Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe) for the financial support.

REFERENCES

Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[FRP Membrane Housing Pressure Vessel for RO Plant]

Brown, E. M; Thompson, C .J.,Good Quality Custom Car Power Window Cable for Ford Car, M.M.(1994),Solar Panel PV System Mc4 Circular Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wedding Tent Cooling System Outdoor Event Air-Con Units]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling. Analytical Biochemistry212,436-445[LC Multistage Pump Mechanical Seal (KLLC)]

Dalev, P.G. and Simeonova, L.S.(1992),Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer. Biotechnology letters 14,6, 531-534[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

Heidemann, E.(1997),Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting.Leder 48,(5),99-104[Acrylic Panel Capacitive Membrane Keypad Switch for Digital Safe Lock]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Viton Coated Half Shaft Wafer Butterfly Valve]

Pre Painted Steel Coil and Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall.Plastic Recycling Centrifugal Dewatering Machine. USP/SP[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nail Stamping Plates Custom Metal Part Sheet Metal Stamping]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PP Food Grade Feeding Bottle Without Handle 240ml Bottle Feeding Baby/Baby Bottles/Baby Water Bottles /Baby Items/Baby Products/Baby Goods/Baby Stuffs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

EAS Anti-Theft Optical Security Tag for Glasses Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Phase Digital Electric Power Meter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway]

EAS Anti-Theft Optical Security Tag for Glasses Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator]

EAS Anti-Theft Optical Security Tag for Glasses Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, Part I.2W 100lm LED Solar Lawn Light for Garden or Yard Lighting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Design Many Styles Fancy Wine Display Rack (6532)]

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),28mm China High Quality Supplier Screw Cannon Cap for Bottle.Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, 175-184[Bendable Led Display Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer]

Whitaker,J.R.(1963),Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure. Analytical Chemistry35(12), 1950-1953[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

4 Gang 1 Way Electrical Fitting Ce CertificateAuthor for correspondence