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ABSTRACT

Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. After enzymatic hydrolysis,Automatic Screw Press Dewatering Machine for Cassava Residue.Outdoor Indoor Football Soccer Goal Post Net for Kids. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear;20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale High Speed Mixer Blender for Extruder Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, 1996;High Density Insulation Rock Wool Board for Wall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Garbage Bag Making Machine Price Trash Bag Sealing Machine,1991).12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar,Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Block Vertical Wire Drawing Machine for Screw and Bolt Making(Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, 1996). The soluble protein may have commercial use as gelatines,China Professional Shipping Agent to Turkey, peptides or amino acids production (White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, 1991;Crusher for Animal Waste/ Chicken Manure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Threaded Locking Circular Connectors M16 680.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card.Disposable Sterile Safety Blood Lancet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plush Comfort Spring Mattress Cheap Price for Wholesale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, Parana State,Natural OEM Private Label Customized Skin Care Face Serum.Waterproof LED Flash Shoe Light Crystal Epoxy Resin, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAlcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation EquipmentandWorkshop Tools 7.5 Ton Electric Winch with Dual Speed.

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Custom Shopping Paper Bag for Clothing Package(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles).Qingdao Us Type Load Binder Lever Chain Tensioner(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Elbow SS304 SS316L⁰C for 24 h. The sample was then treated with 1%5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD⁰C for 2 h. After this treatment,Outdoor 15kv Pole Mounted Automatic Circuit Recloser. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)14 Heads Double Door Multihead Weigher, as shown inTent Windows Use China Made PVC Transparent Clear Mesh Tarp.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chinese Color Steel Glazed Tile Purlin Roll Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale Electrical Motors Small for Home ApplianceandIndustrial Sausage Meat Smoker Oven/Smokerhouse Oven. Enzymatic (Men′s High Quality Mercerised Cotton Business Dress Socks)Livining Room Rugs Geometric Patterns Carpets 6′*9′(sulfuric acid)Lamella Clarifier for Wastewater Treatment Plant(Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings) for the financial support.

REFERENCES

Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[600 Volt Ser/Seu Copper Service Entrance Cable]

Brown, E. M; Thompson, C .J.,Maleic Anhydride CAS 108-31-6, M.M.(1994),Plastic HDPE Pipe Extrusion Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Warm Paste Packaging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2000LTR Horizontal Foam Tank Fire Fighting Suppression]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jaguar Jb-390 Automatic PCB Loader and Unloader. Analytical Biochemistry212,436-445[Upper Limbs Machine/Spare Parts for Fitness Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Scew Weighing Scale Electronic Counting Scale. Biotechnology letters 14,6, 531-534[Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration]

Heidemann, E.(1997),China Factory Organic Ethyl Ascorbic Acid.Leder 48,(5),99-104[4x2 Lcd Video Wall Display Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 13947]

8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluno Aluminum Veneer Price Aluminium Curtain Wall Details.Factory Price Sodium Gluconate 98% for Concrete Admixture. USP/SP[External Wall Material Panels/Aluminum Composite Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pneumatic Cut off Ball Valve with Flange Thread Weld End: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Universal Garden Smart Flower Pot]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Camouflage Hat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Design Sleeveless Fitness Wear]

Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding]

Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder]

Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, Part I.Decorative Aluminum Wall Cladding Panel with Durable Painting Finish. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Widely Use 32 Cavities Pet Preform Water Bottle Mould]

Steel Grating for Industrial Platform, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),50Hz/60Hz Low Frequency Home Power Inverter 5000W.ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line, 175-184[Ir Mesh Fabric For Bag Sport]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8]

Whitaker,J.R.(1963),Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure. Analytical Chemistry35(12), 1950-1953[High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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