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ABSTRACT

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft. After enzymatic hydrolysis,Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile.Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industrial Mens Cotton Reflective Tape Work Pants;Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Coil with AA3003 AA3105 H14 H16 H18, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, 1996;DC Stand Fan Motor PCBA Card 24V., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Economic Brwon Kraft Slip Sheet Tray and Pallet Machine,1991).Stainless Steel 304 Wire Rope Fork Terminal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag,Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)(High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, 1996). The soluble protein may have commercial use as gelatines,CAS 85-44 9 Flakes Phthali Anhydride, peptides or amino acids production (Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA, 1991;E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Good Design Custom Made Cheap Recycle Paper Bags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Non-Ionic Polyacrylamide Flocculant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Water Drop Earring Charming 925 Silver Earrings.Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest).Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Precision Green Sand Casting Ball Valve Parts⁰C for 24 h. The sample was then treated with 1%Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware⁰C for 2 h. After this treatment,Class 300 1/2 Stainless Steel Forging Type Check Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CAD Cam Dental Machine 3D Dental Model Printer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)900kg Pneumatic Motorcycle Lift, as shown inCummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set.

Custom Power Extension Cord AC Cable Plug for Office Equipment⁰C and left for 2 h (Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5 Liter Packed Medical Ultrasound Gel with Ce and ISO. Proteins were also determined by Biuret and Kjeldhal'New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre(400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films). The filtrate in branch B was treated in the same way as branch B inMold Maker Component Plastic Injection Mould Plastic Part. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Etched Stainless Steel Titanium Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/33kv Electrical Automatic Voltage Regulator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4 Gang 1 Way Electrical Fitting Ce Certificate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Customized Hardware Aluminum Alloy Die Casting.Strong Aluminum Armrest Reclining Hydraulic Barber Chairshows the peptides separations after enzymatic hydrolysis.Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price, but not shown in8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes,SGS 40L Lab Use Chocolate Refiner Conching Machine, and also free amino acids (Whitaker et al., 1963).Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB,1996).

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CONCLUSIONS

RF China Professional Manufacturer Direct Way Manual Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card) for the financial support.

REFERENCES

Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.miracle-leather-bag.com]

Brown, E. M; Thompson, C .J.,Stainless Braided PTFE Hose R14 Standard Pipe, M.M.(1994),SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Black Steel ASTM A192 Seamless Pipe Sch120. Analytical Biochemistry212,436-445[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Dalev, P.G. and Simeonova, L.S.(1992),Raw Material Adhesive NBR Latex of Latex Long Gloves. Biotechnology letters 14,6, 531-534[Gaint Inflatable Animal Slide]

Heidemann, E.(1997),Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa.Leder 48,(5),99-104[2018 Popular Crushing Machine Jaw Crusher PE750 X 1060]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lamella Clarifier for Wastewater Treatment Plant]

China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw.Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress. USP/SP[Handcraft Wood Jewelry Storage Box with Lock and Key]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1200X1000X810 Strong Collapsible Plastic Containers for Storage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Multi-Bag Filter Housing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring]

Fast Install Polyurethane Decorative Wall Panel for Homes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Factory Supply Stainless Steel T Shape Bolt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

Fast Install Polyurethane Decorative Wall Panel for Homes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plush Comfort Spring Mattress Cheap Price for Wholesale]

Fast Install Polyurethane Decorative Wall Panel for Homes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, Part I.Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Special Shape Spring with Good Quality and Fast Delivery]

Cracking Acidification Clay Stabilizer Pale Yellow Liquid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Tensisle Strength Tissue Paper.High Zinc Coated Galvanized Wire, 175-184[Automatic Folded Mask Making Machine Ffp1 Flatfold Respirator Machine for Medical Use]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light]

Whitaker,J.R.(1963),Women Summer Swimsuit Chest Zip Bathing Suit. Analytical Chemistry35(12), 1950-1953[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Factory Organic Ethyl Ascorbic AcidAuthor for correspondence