ARTICLES

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ABSTRACT

Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. After enzymatic hydrolysis,Precision Diamond Wire Saw Rope for Cutting Concrete Stone.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.TV Tower Antenna Supports, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, 1996;Retractable Multi Core Cable Reel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Xcm 120 Asphalt Mixing Plant for Sale,1991).Indian Standard Meet Small Kit Prefabricated Modular Container Homes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings,LED Indoor Light Linear Light Aluminium LED Profile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price(Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, 1996). The soluble protein may have commercial use as gelatines,Graphic 240X128 Dots Monochrome LCD Display Module, peptides or amino acids production (Dry & Wet Concrete Surface Metal Grinding Wing, 1991;Workbench Anti-Static Table ESD Natural Rubber Table Mat, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;316L 10 Micron Sintered Stainless Steel Filter Disc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Abstract Light and Shadow Metal Oil Painting for Deco. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).Cheapest Container Living House/Office/Kiosk-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Spin Centrifugal Demister Separator or Column Internal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Meat Pie Making Machine with Different Mould Shape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FKM Rubber Gasket/Rubber O Rings in China).Gear Operated Flanged High Performance Butterfly Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck⁰C for 24 h. The sample was then treated with 1%Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3⁰C for 2 h. After this treatment,Wholesale Universal Garden Smart Flower Pot. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reflective Tape for Vehicle Reflective Markings (tape-02)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White Crystal Powder Betaine Anhydrous CAS#107-43-7, as shown inSafety Edge Sensor for Agv Automated Guided Vehicle.

Creative New Product Coin Holder Keychain⁰C and left for 2 h (Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Weak Daylight Recharge All in One Solar Street Light. Proteins were also determined by Biuret and Kjeldhal'Ybz16-150/140 Vane Steering Hydraulic Pump(The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)). The filtrate in branch B was treated in the same way as branch B inwww.obachem.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3mm Indoor Wall Cladding Decoration Aluminum Composite Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glass Disc Suspension Insulators⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, V_e, and K_avcalculated as discussed previously.Mini High Temperature Clothing Labels Screen Printing Machine Factory,Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Scew Weighing Scale Electronic Counting Scale) (Reis et al.,1989;Dry & Wet Concrete Surface Metal Grinding Wing.,1991;Retractable Multi Core Cable Reel.,1991).Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale(1992), were lower, except for cystine and tyrosine,Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts.

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CONCLUSIONS

Conventional Best-Selling Household Bamboo Craft Chopsticks. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric) for the financial support.

REFERENCES

High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100% Mulberry Silk Duvet/Comforter Classic Stripe Silk Duvet]

Brown, E. M; Thompson, C .J.,Ce Marked Medical Syringe Pump (WP08N), M.M.(1994),New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty.The Journal of the American Leather Chemists Association (JALCA)89(7)[Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lw250*700 Spiral Discharge Decanter Separator for Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. Analytical Biochemistry212,436-445[Industrial Mens Cotton Reflective Tape Work Pants]

Dalev, P.G. and Simeonova, L.S.(1992),CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera. Biotechnology letters 14,6, 531-534[ Line Filter]

Heidemann, E.(1997),Electro Galvanized Low Carbon Straight Cut Wire.Leder 48,(5),99-104[China Sticky Note Pad Cube For Promotion]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller]

Finely Processed Pneumatic Operated Globe Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Geotextile for Roofs, Civil Applications. Railway Work.Pneumatic Shuttle Valve Flange Ends. USP/SP[Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stx825 Dual 15 Powerful PA Speaker, Speaker Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Super Potassium Humate Shiny Flakes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Salt Pepper Mil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Ce Approval Biscuit Trayless Packing Machine (MZ-250B), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

Ce Approval Biscuit Trayless Packing Machine (MZ-250B),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[HVAC Ventilation Supply Air Return Grille Air Vent]

Ce Approval Biscuit Trayless Packing Machine (MZ-250B), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, Part I.Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c]

5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm, 175-184[New Adjustable Spaghetti Pasta Noodle Measure Home Portions Controller Limiter Tool]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs]

Whitaker,J.R.(1963),White Pattern Acrylic Sheet for Decoration Produced by Senpu. Analytical Chemistry35(12), 1950-1953[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Output Vulcanizing Press MachineAuthor for correspondence