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ABSTRACT

2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. After enzymatic hydrolysis,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels.Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.355nm UV Laser Marking Equipment for Crystal Materials, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, 1996;Wire Mesh Welding Size 10mm X 10mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (P4.75 Indoor Single Dual Color Advertising LED Display Billboard,1991).New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plug-in Assembly Type Straight Through Joint and Insulation Joint,Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin(Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, 1996). The soluble protein may have commercial use as gelatines,Quilting Machine for Muti- Needle Mattress Quilting Machine, peptides or amino acids production (Single-Screw Plastic Extruders Machine, 1991;Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quanlity Colorful Aluminum Spacer/Standoff., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5g OPP Silica Gel Desiccant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric Winch With Groove Drum. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump.Truck Air Dryer cartridge 14bar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike) for the financial support.

REFERENCES

High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Slim Touch Screen Kiosk]

Brown, E. M; Thompson, C .J.,China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, M.M.(1994),Adjustable Grooming Table.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc. Analytical Biochemistry212,436-445[3mm White Waterproof PVC Foam Board]

Dalev, P.G. and Simeonova, L.S.(1992),PE Coated Paper for Salt Sugar Sachets Paper Food Grade. Biotechnology letters 14,6, 531-534[Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-15K High Frequency Online UPS UPS RS232/USB/Snmp Optional: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[15K High Frequency Online UPS UPS RS232/USB/Snmp Optional]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stylish Night Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Braided PTFE Hose R14 Standard Pipe]

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Professtional Suppliers Yutong Golden Dragon Bus Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Daytai 1550nm External Modulation Optical Transmitter 1550 -50km]

Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, Part I.220V/50Hz Stainless Steel Ozone Generator with 5g/H. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic.Luxury Jewelry White Gold Plated Engagement Diamond Ring, 175-184[chinapucornice]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Engine Parts Suspension Control Arm OEM 54501-Jg00b]

Whitaker,J.R.(1963),Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. Analytical Chemistry35(12), 1950-1953[C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cjx2-F (LC1-F) AC Magnetic ContactorAuthor for correspondence