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ABSTRACT

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Key words;Oral Thermometer;Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wood Fireplace Double Skin Chimney Flue Pipes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, 1996;High Sensitivity Ground Metal Detector Searching for Treasure., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminium Profile Bending Machine for Insulating Glass,1991).Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,304 Thick Wall Pressure Vessel Heat Exchanger Pipe,High Quality Betaine Anhydrous with Best Price107-43-7,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards(PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, 1996). The soluble protein may have commercial use as gelatines,Extra Heavy Wall Thickness Seamless Tubes, peptides or amino acids production (Shrink Sleeves Labels for Packaging Labeling, 1991;IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Mould Dongguan Molding Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer.PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality Banded Type Tee(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

FKM Rubber Gasket/Rubber O Rings in China, Parana State,Barricade Tape.Inclined Waste Water Treatment Lamella Clarifier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMore Cavities Plastic Mould DesignandYuchai 400kw Diesel Generator Set.

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33kv 100kVA Dry-Type Distribution Transformer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chinese Color Steel Glazed Tile Purlin Roll Forming Machine).Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit⁰C for 24 h. The sample was then treated with 1%5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring⁰C for 2 h. After this treatment,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc 's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Export Copper Foot Tap/Valve (HFV-001), as shown in150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNiu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.RFID Blocking Leather Slim Zipper Credit Card Holder Wallet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2017 New Design Vinyl 3D Stone Wallpaper Factory in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Small Paper Gift Box with Magnet Closure for E-Cigarette, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1080W Most Powerful LED Flood Light Fixtures. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles) for the financial support.

REFERENCES

OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Eco-Friendly Fry Steamer]

Brown, E. M; Thompson, C .J.,Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, M.M.(1994),High Quality Bucket Segment Bolt 6V0937.The Journal of the American Leather Chemists Association (JALCA)89(7)[Crane Hook Qtz250 (7032) with 70m Jib Length]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture. Analytical Biochemistry212,436-445[Men Casual Short Pants Custom Quality Running Sports Wear Shorts]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Server Rack Network Data Cabinet. Biotechnology letters 14,6, 531-534[Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course]

Heidemann, E.(1997),Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera.Leder 48,(5),99-104[Hydraulic Cylinders Saskatchewan]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Injection Molding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Women Summer Swimsuit Chest Zip Bathing Suit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Western-Style Commercial Induction Flat Stew-Pot Cooker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Steel Pipe ASTM A106 A53-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Finely Processed Pneumatic Operated Globe Valve]

OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision Green Sand Casting Ball Valve Parts]

OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel, Part I.Peptides Powder Ghrp 2 for Muscle Building with Super Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Spped Weaving Machine 6]

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),EPDM, PVC Car or Door Rubber Sealing Strip.Black Color Tube Eyelet Lift Gas Spring, 175-184[Spherical Yttrium Oxide]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Car Parking or Access Control 2.4 GHz Active RFID Card]

Whitaker,J.R.(1963),Safety Edge Sensor for Agv Automated Guided Vehicle. Analytical Chemistry35(12), 1950-1953[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion LineAuthor for correspondence