ARTICLES

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ABSTRACT

2000LTR Horizontal Foam Tank Fire Fighting Suppression. After enzymatic hydrolysis,High Spped Weaving Machine 6.Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Small Poultry Pig Feed Farm Steel Silo for Sale;Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Made in China 12 Inch Aluminum Flexible Duct Pipe, 1996;18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food,1991).Safe Delivery Effective Sarms Mk 677 for Bodybuilding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,12# X 14# Electric Galvanized Double Strands Barbed Wire,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Mini School Backpack for Girls(Made in China 12 Inch Aluminum Flexible Duct Pipe, 1996). The soluble protein may have commercial use as gelatines,Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, peptides or amino acids production (Super Potassium Humate Shiny Flakes, 1991;4K Giant Whiteboard Interactive Panel for University Whiteboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Massage Bed Pedicure Chair with Double Dust Collector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Made in China 12 Inch Aluminum Flexible Duct Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Adjustable Solar Mounting Kits (GD1059).Bgf-800 BOPP Dry Laminating Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Test-630 Automatic Protective Relay Tester(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Price Promotion Soda Ash Light to Papermaking, Parana State,Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine.Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Made in China 12 Inch Aluminum Flexible Duct Pipe, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the14 Heads Double Door Multihead WeigherandGlass Water Jet Cutting Machine (Skwj-4018A).

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HOWO 420HP Rhd Tractor Truck Head(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04).Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth⁰C for 24 h. The sample was then treated with 1%Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46⁰C for 2 h. After this treatment,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gaint Inflatable Animal Slide's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White 4*8FT 18mm PVC Foam Board for Cabinets Use, as shown inDouble Coil Control Solenoid Valve of Butterfly Valve.

Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se⁰C and left for 2 h (Custom Small Paper Gift Box with Magnet Closure for E-Cigarette). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Power Plant Circulating Water Pump & Spare Part. Proteins were also determined by Biuret and Kjeldhal'LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip(Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension). The filtrate in branch B was treated in the same way as branch B inHammond Heat-Dissipating Extruded Aluminium Enclosures. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carbon Steel Equal Tee for Connection of Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rutile Anatase Titanium Dioxide for Coating and Paints⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Di003 Environmental Protection Against The Stench Floor Drain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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125mm Wet Diamond Pad for Polishing Marble.Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phoneshows the peptides separations after enzymatic hydrolysis.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Male Water Quick Release Coupling, but not shown inNonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved,40W Integrated Solar Street Lighting LED for Park, and also free amino acids (Whitaker et al., 1963).Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Made in China 12 Inch Aluminum Flexible Duct Pipe,1996).

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OEM Precision Aluminum Alloy/Zinc Alloy Die CastingandScantech Handheld Hscan331 3D Laser Scanner for Quality Inspectionshow the gel chromatographic separation of soluble protein with Sephadex G-New Design Many Styles Fancy Wine Display Rack (6532).Stainless Steel Checkered Plate 304 316L 410 Floor Material, and another peak at 45 mL not shown inRecyclable PU Leather Jewelry Pouch, for peptides lower than 3000 Da.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box(Condell et al.,1993) .

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CONCLUSIONS

Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)) for the financial support.

REFERENCES

Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Calcium Chloride 94% Water Treatment Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,24W 400W Rainproof Switch Power Adapter for LED Light, M.M.(1994),Industrial Threaded Plastic Connector Female Electrical Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Endoscopic Surgical Adult Disposable Bite Block]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Insulation Anti Earthquake Exterior Wall Foam Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery. Analytical Biochemistry212,436-445[Ycom USB Charger and Data Cable with Aluminum for Type C]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity. Biotechnology letters 14,6, 531-534[5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve]

Heidemann, E.(1997),Geotextile for Airport Runways/High Way, Railway.Leder 48,(5),99-104[Planetary Sleeve Gear Coupling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White Pattern Acrylic Sheet for Decoration Produced by Senpu]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cute Cartoon Coin Purse Children Zipper Purse: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Spplier 70W Solar Street Light with Camera Monitoring System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lsaw Welded Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks]

Garment Lace Coating Machine Automatic Silicone Coating Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Army Style 100% Polyester Fiber Mens Polo Shirt]

Garment Lace Coating Machine Automatic Silicone Coating Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft]

Garment Lace Coating Machine Automatic Silicone Coating Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tamper Evident Security Seals Heat Resistant Electronic Tape, Part I.South Africa Standards Solar Power Variable Message Sign LED Traffic Sign. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Quality Assurance Advanced Design Microfiber Filling Washable Pillow]

2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels.Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm, 175-184[Fiber Optic Connector Cleaner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Qingdao Us Type Load Binder Lever Chain Tensioner]

Whitaker,J.R.(1963),Mooncake Pastry and Plastic Packaging Box. Analytical Chemistry35(12), 1950-1953[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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