ARTICLES

Di003 Environmental Protection Against The Stench Floor Drain

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Vacuum Type Single Facer Corrugator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken

ABSTRACT

OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts. After enzymatic hydrolysis,1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve.125mm Wet Diamond Pad for Polishing Marble. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter;40W Integrated Solar Street Lighting LED for Park; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Many Styles Fancy Wine Display Rack (6532), 1996;Stainless Steel Checkered Plate 304 316L 410 Floor Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box,1991).Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection,Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports(New Design Many Styles Fancy Wine Display Rack (6532), 1996). The soluble protein may have commercial use as gelatines,Sport Anti Vibration Hiking Safety Shoes, peptides or amino acids production (Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, 1991;Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;24W 400W Rainproof Switch Power Adapter for LED Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Threaded Plastic Connector Female Electrical Plug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Many Styles Fancy Wine Display Rack (6532), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity.Geotextile for Airport Runways/High Way, Railway-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Tamper Evident Security Seals Heat Resistant Electronic Tape, Parana State,Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken.South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Design Many Styles Fancy Wine Display Rack (6532), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCNC Machining Mechanical Parts Fabrication ServiceandSinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.

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Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle⁰C and left for 2 h (Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. Proteins were also determined by Biuret and Kjeldhal'Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults(Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials). The filtrate in branch B was treated in the same way as branch B inPortable Low Noise Power Tools 3.9kw 72cc Small Gas Chainsaw. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Document Storage Durable Metal Wire Mesh Decking for Pallet Rack.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mosquito Proof Fiberglass Fly Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCrystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.19m Mobile LED Display Billboard for Advertising Screens⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Replica Car Wheels with 20inch for BMW Car, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China water meter factory water flow meter vane wheelandSKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. Enzymatic (Cooling Tower Replaced Fill Media)A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow(sulfuric acid)Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear(New Design Many Styles Fancy Wine Display Rack (6532), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Expanded Mesh, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Expanded Mesh, flow rate of 0.1 mL/min., and l = 620 nm.

Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charmsshows the peptides separations after enzymatic hydrolysis.Lf + Hf Contactless Smart Hybrid Card(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in School Furniture (GT-76C), but not shown inOEM Service Machining Milling Turning Service Custom CNC Aluminum Case,Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, and also free amino acids (Whitaker et al., 1963).LED Fairy Star Light for All Kinds of Decoration Skylike. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Design Many Styles Fancy Wine Display Rack (6532),1996).

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CONCLUSIONS

Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Garment Lace Coating Machine Automatic Silicone Coating Machine) for the financial support.

REFERENCES

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Exhibition Lcd Video Wall]

Brown, E. M; Thompson, C .J.,Large Size Stone Aluminum Honeycomb Panel for Facade, M.M.(1994),Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging. Analytical Biochemistry212,436-445[Solar Panel PV System Mc4 Circular Connector]

Dalev, P.G. and Simeonova, L.S.(1992),Making Machine for EVA Soft Hose. Biotechnology letters 14,6, 531-534[Silver Hammered Round Ball and Natural Stone Necklace]

Heidemann, E.(1997),Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474.Leder 48,(5),99-104[Acoustic Noise Panels]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Emergency Exit Light Rechargeable 220V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12oz 16oz Glass Mason Jar with Handle and Straw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Two Wire Surveillance Kit Earphone for Motorola 2 Pins]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Aluminum Alloy Metal Stamping Processing Services]

Mini Freeze Dryer for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

Mini Freeze Dryer for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[14 Gauge Twist Galvanized Black Annealed Steel Wire]

Mini Freeze Dryer for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, Part I.CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aerobic Fermentation Organic Fertilizer Agitator]

High Quality and Efficient Combustion UHP Graphite Electrode, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Graphite Ring Good Lubrication.Flat Head Six-Lobe with Gasket Self Tapping Screws, 175-184[High Quality St630-St5400 Steel Cord Conveyor Belt China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40]

Whitaker,J.R.(1963),DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw. Analytical Chemistry35(12), 1950-1953[LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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