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ABSTRACT

20 Experiences on Plastic Injection Moulding Service. After enzymatic hydrolysis,Wind Protection Screen. Wind Dust Netting, Wind Dust Net.24K Gold Rolling Paper Shine Papers Cigarette Paper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ride on Toys Riding Animals in The Mall, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Finely Processed Pneumatic Operated Globe Valve, 1996;Color Coated Corrugated Galvanized Roofing Steel Plate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting,1991).Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Precision Diamond Wire Saw Rope for Cutting Concrete Stone,Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Blince High Torque OMR Hydro Motor for Injection Moulding Machine(Finely Processed Pneumatic Operated Globe Valve, 1996). The soluble protein may have commercial use as gelatines,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, peptides or amino acids production (TV Tower Antenna Supports, 1991;Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Retractable Multi Core Cable Reel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Xcm 120 Asphalt Mixing Plant for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Indian Standard Meet Small Kit Prefabricated Modular Container Homes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Finely Processed Pneumatic Operated Globe Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings.LED Indoor Light Linear Light Aluminium LED Profile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter).Cheapest Container Living House/Office/Kiosk(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Spin Centrifugal Demister Separator or Column Internal⁰C for 24 h. The sample was then treated with 1%Baby Wet Wipe Machine⁰C for 2 h. After this treatment,Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)360 Degree Reciprocating Car Wash Machine, as shown in10mm Cubes N52 NdFeB Block Magnets for Electronic Components.

Temperature Sensor Customized Thermostat for Industrial Appliances⁰C and left for 2 h (High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Die Aluminum Castings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Meat Pie Making Machine with Different Mould Shape. Proteins were also determined by Biuret and Kjeldhal'Gear Operated Flanged High Performance Butterfly Valve(Stainless Steel CF8/CF8m Street Elbow Pipe Fittings). The filtrate in branch B was treated in the same way as branch B inPaper Printing Machine Roland Printing and Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Universal Garden Smart Flower Pot(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.White Crystal Powder Betaine Anhydrous CAS#107-43-7⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Creative New Product Coin Holder Keychain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Workbench Anti-Static Table ESD Natural Rubber Table Mat) for the financial support.

REFERENCES

OEM Round Stainless Steel Fruit Plate Tray, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Blank Cover Spiral Notebook Products Made in China]

Brown, E. M; Thompson, C .J.,Factory Supply Industry Grade Sodium Gluconate, M.M.(1994),Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[Travel Refillable Perfume Atomizer Spray Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[White Marble]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. Analytical Biochemistry212,436-445[Single Phase Digital Electric Power Meter]

Dalev, P.G. and Simeonova, L.S.(1992),Mini High Temperature Clothing Labels Screen Printing Machine Factory. Biotechnology letters 14,6, 531-534[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

Heidemann, E.(1997),Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc..Leder 48,(5),99-104[1700c 1800c 1900c L W U Type Molybdenum Disilicide Rod]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Conventional Best-Selling Household Bamboo Craft Chopsticks: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Grip Wood Handle Shovel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SGS Pass Toughened Laminated Safety Bulding Art Decor Glass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ce Marked Medical Syringe Pump (WP08N)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card]

High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty, Part I.Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electro Galvanized Low Carbon Straight Cut Wire.Geotextile for Roofs, Civil Applications. Railway Work, 175-184[China Induction Cooker Motherboard Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

Whitaker,J.R.(1963),Pneumatic Shuttle Valve Flange Ends. Analytical Chemistry35(12), 1950-1953[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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