ARTICLES

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ABSTRACT

Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2. After enzymatic hydrolysis,Lf + Hf Contactless Smart Hybrid Card.Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Linear Vibrator Screener, for Granule and Powder Selection and Classification, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, 1996;Children Hardcover Book Printing Services with Free Printing File Checking., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid,1991).Global Brands 10 Year Cheaper Hook & Loop Tape Male Side. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw,Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt(Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, 1996). The soluble protein may have commercial use as gelatines,25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, peptides or amino acids production (Large Size Stone Aluminum Honeycomb Panel for Facade, 1991;Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Making Machine for EVA Soft Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Efficiency Air Cooled Air Source Water Chiller Heat Pump.Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12oz 16oz Glass Mason Jar with Handle and Straw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Mini Freeze Dryer for SaleAlcalase2.5 L DX,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

High Quality and Efficient Combustion UHP Graphite Electrode, Parana State,Stainless Steel Expanded Mesh.Graphite Ring Good Lubrication, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAfraic Market Mobile Food Vending CartandOil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe.

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Flat Head Six-Lobe with Gasket Self Tapping Screws(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Machining Mechanical Parts Fabrication Service).DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile⁰C for 24 h. The sample was then treated with 1%0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting⁰C for 2 h. After this treatment,Weiying Full Computerized Plain and Invisible Sock knitting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Double Cereal Airtight Food Dispenser's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Food Packaging Paper Soup Bowl with Lids, as shown inSinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.

500ml PP Sport Water Bottle with Ball⁰C and left for 2 h (23m 4 R Arms 2017 New Design Concrete Placing Boom). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stamping Fine Blanking Sheet Metal Fabrication Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins. Proteins were also determined by Biuret and Kjeldhal'Trade Assurance Beautiful Silk Lace Closure(Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure). The filtrate in branch B was treated in the same way as branch B inBaby Heat Sensitive Spoon Fork Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Needle Rollers for Bearings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heavy Duty Steel Warehouse Storage Pallet Rack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCoil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Bulk Price Yk-11 Powder for Bodybuilding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fully Automatic Digital Pid Temperature Controller.Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/Vshows the peptides separations after enzymatic hydrolysis.High Quality Anti-Corrosion Fiberglass Sheet and Roll(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, but not shown inChina water meter factory water flow meter vane wheel,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, and also free amino acids (Whitaker et al., 1963).Full-Automatic Folding Napkin Paper Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket,1996).

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School Furniture (GT-76C)andOEM Service Machining Milling Turning Service Custom CNC Aluminum Caseshow the gel chromatographic separation of soluble protein with Sephadex G-Customized Frame Mount Highway Pegs Enclosure Parts.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, and another peak at 45 mL not shown inFull Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies, for peptides lower than 3000 Da.10mm Tempered Bathroom Glass Safety Glass for Building(Condell et al.,1993) .

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3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, V_e, and K_avcalculated as discussed previously.Cattle Feed Factory Used Dust Collector and Filter,Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader) (Reis et al.,1989;Large Size Stone Aluminum Honeycomb Panel for Facade.,1991;Children Hardcover Book Printing Services with Free Printing File Checking.,1991).Group 152cm Picnic Folding in Half Table(1992), were lower, except for cystine and tyrosine,Tactile Custom Branded Membrane Switch Keyboard with Foil.

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CONCLUSIONS

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure) for the financial support.

REFERENCES

Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Women Eco-Friendly Racerback Wording Printed Ombre Vegan Fitness Sportwear Tank Top]

Brown, E. M; Thompson, C .J.,Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, M.M.(1994),Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cleaning Lint Free Microfiber Mop]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Queen Mattress with Visco Memory Foam and Latex]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Australia Two Pins Power Cord with SAA Certification. Analytical Biochemistry212,436-445[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

Dalev, P.G. and Simeonova, L.S.(1992),87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger. Biotechnology letters 14,6, 531-534[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Heidemann, E.(1997),Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%.Leder 48,(5),99-104[Booth Cat Tubuh Otomatis]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net.Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. USP/SP[Solar Panel PV System Mc4 Circular Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silver Hammered Round Ball and Natural Stone Necklace]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Steel Pipes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable]

Pickled Cucumber Production Line/Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rectangular Lunch Tin Box with Handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Emergency Exit Light Rechargeable 220V]

Pickled Cucumber Production Line/Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting]

Pickled Cucumber Production Line/Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Inflatable Cheering Stick for Promotional Gift, Part I.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Aluminum Alloy Metal Stamping Processing Services]

Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2.Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, 175-184[17keys]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

Whitaker,J.R.(1963),2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. Analytical Chemistry35(12), 1950-1953[14 Gauge Twist Galvanized Black Annealed Steel Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aerobic Fermentation Organic Fertilizer AgitatorAuthor for correspondence