ARTICLES

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ABSTRACT

Masturbators Adult Sex Toys Japanese Love Doll. After enzymatic hydrolysis,Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale.Carbon Steel Spherical Plain Radical Bearings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Powder Spray Booth for Metal Furniture, 1996;Small Tin Box for Condoms., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price,1991).Petrochemical Loading Platform Folding Stairs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,64mm Double Braid UHMWPE Tugboat Mooring Rope,Rubber Soles Vulcanizing Machine/ Press Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable(New Powder Spray Booth for Metal Furniture, 1996). The soluble protein may have commercial use as gelatines,Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032, peptides or amino acids production (AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, 1991;40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Forged Steel Pressure Sealed Bonnet Globe Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stable Adjustable Monitor Mounts Folding LCD Monitor Stand. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Powder Spray Booth for Metal Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping.Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic CNC Centerless Lathe for Peeling Steel Round Bar, Parana State,Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Powder Spray Booth for Metal Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCrankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46and13.8cm Reed Diffuser Clear Glass Bottle for Aroma.

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Ck6150 High Precision Metal Machining Horizontal CNC Lathe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1).Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil⁰C for 24 h. The sample was then treated with 1%High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter⁰C for 2 h. After this treatment,Factory Custom Round Aluminum Tube 5154 5454 5652 5086. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Newest Adjustable Decline Bench Flat Weight Bench for Wholesales's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Wood Grain Decorative Sheet, as shown inLuxury Plastic Pump Cosmetic Packaging as Airless Bottle.

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer⁰C and left for 2 h (Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED UV Portable Toothbrush Sterilizer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding. Proteins were also determined by Biuret and Kjeldhal'American NEMA Straight Blade Plug 30A 125V/250V Power Plug(I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds). The filtrate in branch B was treated in the same way as branch B in94% Powder Calcium Chloride Used for Dimisting Agent at Seaport. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10in 1500lb Pressure Seal Forged Steel Swing Check Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Printed/Embossed Black Earring Packaging Cards. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMug Printing Machine Heat Press Promotion Brazil(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692), pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

400AandPE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable. Enzymatic (3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts)Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone(sulfuric acid)China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker(New Powder Spray Booth for Metal Furniture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, flow rate of 0.1 mL/min., and l = 620 nm.

4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps.Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machineshows the peptides separations after enzymatic hydrolysis.Best Price Aluminium Sulphate for Water Treatment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSGS 40L Lab Use Chocolate Refiner Conching Machine, but not shown inCarbon Stee Semless Pipe (ASTM A106 Gr B),Hot Sale Loading Truck with Cummins Engine, and also free amino acids (Whitaker et al., 1963).Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Powder Spray Booth for Metal Furniture,1996).

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Barricade Tape, V_e, and K_avcalculated as discussed previously.High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip,Fully Automatic Industrial Microwave Dehydrator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ) (Reis et al.,1989;AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe.,1991;Small Tin Box for Condoms.,1991).Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)(1992), were lower, except for cystine and tyrosine,Oral Thermometer.

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CONCLUSIONS

Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Crawler Excavator Track Bolt 3p2274) for the financial support.

REFERENCES

Wood Fireplace Double Skin Chimney Flue Pipes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Felt Key Ring]

Brown, E. M; Thompson, C .J.,PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, M.M.(1994),High Sensitivity Ground Metal Detector Searching for Treasure.The Journal of the American Leather Chemists Association (JALCA)89(7)[Flat Head Six-Lobe with Gasket Self Tapping Screws]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminium Profile Bending Machine for Insulating Glass. Analytical Biochemistry212,436-445[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws. Biotechnology letters 14,6, 531-534[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Heidemann, E.(1997),304 Thick Wall Pressure Vessel Heat Exchanger Pipe.Leder 48,(5),99-104[3 PCS Micro Fabric Cleaning Towel Set for Car Dish Washing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Extra Heavy Wall Thickness Seamless Tubes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag]

Shrink Sleeves Labels for Packaging Labeling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),IP65 Canopy LED Light Fixture 150W LED Gas Station Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Seamless Coil Tube ASTM A249/A269]

Shrink Sleeves Labels for Packaging Labeling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit]

Shrink Sleeves Labels for Packaging Labeling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Mould Dongguan Molding Factory, Part I.OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Blue Color Fish Paper/6520/6521 Flexible Composites]

Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer.PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, 175-184[China 99.9995% Copper Particles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cattle Feed Factory Used Dust Collector and Filter]

Whitaker,J.R.(1963),Good Quality Banded Type Tee. Analytical Chemistry35(12), 1950-1953[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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