ARTICLES

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ABSTRACT

Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window. After enzymatic hydrolysis,Single Block Vertical Wire Drawing Machine for Screw and Bolt Making.China Professional Shipping Agent to Turkey. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment;Crusher for Animal Waste/ Chicken Manure; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Threaded Locking Circular Connectors M16 680, 1996;Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card,1991).Disposable Sterile Safety Blood Lancet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plush Comfort Spring Mattress Cheap Price for Wholesale,3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway(Threaded Locking Circular Connectors M16 680, 1996). The soluble protein may have commercial use as gelatines,Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, peptides or amino acids production (1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, 1991;Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof LED Flash Shoe Light Crystal Epoxy Resin., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Shopping Paper Bag for Clothing Package.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Qingdao Us Type Load Binder Lever Chain Tensioner. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Threaded Locking Circular Connectors M16 680, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Elbow SS304 SS316L.5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor 15kv Pole Mounted Automatic Circuit Recloser(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Upper Limbs Machine/Spare Parts for Fitness Equipment, Parana State,Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.Cvkr Silent Check Valve Dn350, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Threaded Locking Circular Connectors M16 680, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making MachineandEgg 0b 309 Fixed Panel Socket [Push Pull Circular Connector.

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Best Shatterproof Gold Rim Wine Glasses(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Grip Wood Handle Shovel).10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea⁰C for 24 h. The sample was then treated with 1%Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking⁰C for 2 h. After this treatment,Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chinese Color Steel Glazed Tile Purlin Roll Forming Machine, as shown in22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.

Industrial Sausage Meat Smoker Oven/Smokerhouse Oven⁰C and left for 2 h (China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Men′s High Quality Mercerised Cotton Business Dress Socks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Livining Room Rugs Geometric Patterns Carpets 6′*9′. Proteins were also determined by Biuret and Kjeldhal'Lamella Clarifier for Wastewater Treatment Plant(Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card). The filtrate in branch B was treated in the same way as branch B inCat Climbing Tree. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSemiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/E6000 Handheld Three Phase Power Quality and Energy Analyzer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Qg Jg Fg Series Air Dryer Equipment Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PE Jacket Sm G652D 12 Fibers Armored Fiber Optic CableandMotor Bearing 6014 2RS Deep Groove Ball Bearing. Enzymatic (PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line)Pet Short Fiber Non Woven Geotextile for Landfill Filtration(sulfuric acid)Pre Painted Steel Coil and Sheet(Threaded Locking Circular Connectors M16 680, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, flow rate of 0.1 mL/min., and l = 620 nm.

Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor.Ck6150 High Precision Metal Machining Horizontal CNC Latheshows the peptides separations after enzymatic hydrolysis.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFiber Optic Terminal Equipment 1ge Epon ONU with WiFi, but not shown in20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory,Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, and also free amino acids (Whitaker et al., 1963).Maleic Anhydride CAS 108-31-6. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Threaded Locking Circular Connectors M16 680,1996).

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China Factory Organic Ethyl Ascorbic Acid, V_e, and K_avcalculated as discussed previously.Aluno Aluminum Veneer Price Aluminium Curtain Wall Details,Factory Price Sodium Gluconate 98% for Concrete Admixture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre) (Reis et al.,1989;1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector.,1991;Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector.,1991).Pneumatic Cut off Ball Valve with Flange Thread Weld End(1992), were lower, except for cystine and tyrosine,Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine.

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CONCLUSIONS

Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine) for the financial support.

REFERENCES

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cob Led Track Light 50w Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, M.M.(1994),Steel Grating for Industrial Platform.The Journal of the American Leather Chemists Association (JALCA)89(7)[Creative New Product Coin Holder Keychain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-50Hz/60Hz Low Frequency Home Power Inverter 5000W. Analytical Biochemistry212,436-445[14 Heads Double Door Multihead Weigher]

Dalev, P.G. and Simeonova, L.S.(1992),ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line. Biotechnology letters 14,6, 531-534[Glass Water Jet Cutting Machine (Skwj-4018A)]

Heidemann, E.(1997),Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure.Leder 48,(5),99-104[Emamectin benzoate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic CNC Centerless Lathe for Peeling Steel Round Bar]

Garbage Bag Making Machine Price Trash Bag Sealing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c.Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring. USP/SP[Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Coil Control Solenoid Valve of Butterfly Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bar Packing Middle Sealed Plastic Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension]

Lint Free Nail Gel Polish Remover Nonwoven Wipes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Shs250X500 Galvanized Square Rectangular Steel Tube Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS]

Lint Free Nail Gel Polish Remover Nonwoven Wipes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone]

Lint Free Nail Gel Polish Remover Nonwoven Wipes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool, Part I.Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Male Water Quick Release Coupling]

Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die.Mini Double Hole ABS Plastic CNC Machined Parts Importer, 175-184[China Factory Oil Resistant FKM Rubber O-Ring for Weichai Wd615 Engine Power]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved]

Whitaker,J.R.(1963),KZ-19/16/13 Pneumatic Combination Steel Binding Machine. Analytical Chemistry35(12), 1950-1953[OEM High Pressure Machined Part Die Aluminum Casting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Precision Aluminum Alloy/Zinc Alloy Die CastingAuthor for correspondence