ARTICLES

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ABSTRACT

Colorful Electric English Funny Music Story Module Printing Book. After enzymatic hydrolysis,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine.IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tilt-and-Turn-with-Hidden-Hinge;Automatic Soft PVC Granule Used for Refrigerator Door Gasket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0.45um Dia 47mm PVDF Filter Membrane, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Commecial Use Watch Display Cabinet for Watch Store Interior Design, 1996;Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Slotted Round Head Aluminum Self Tapping Screw,1991).Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Classic Black Color Commercial Glass Door Multi Deck Refrigerator,MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hotbar Reflow Machine for FPC of Camera(Commecial Use Watch Display Cabinet for Watch Store Interior Design, 1996). The soluble protein may have commercial use as gelatines,Full Viton Coated Half Shaft Wafer Butterfly Valve, peptides or amino acids production (Claus Process Catalyst 4-6 mm, 1991;4 Different Ball Silicone Ice Cube Maker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Washable Durable Hotel Polyester Banquet Table Cloths., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Commecial Use Watch Display Cabinet for Watch Store Interior Design, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aerobic Fermentation Organic Fertilizer Agitator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Recyclable PU Leather Jewelry Pouch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring).Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Activated Alumina Used for Catalyst Carrier in Chemical Industry⁰C for 24 h. The sample was then treated with 1%High Quality 45W 50W Commercial Recessed COB LED Downlight⁰C for 2 h. After this treatment,Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, as shown inPneumatic Cut off Ball Valve with Flange Thread Weld End.

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer⁰C and left for 2 h (Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Auto Corrugated Carton Box Paperboard Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Waterproof BNC Connector for Industry. Proteins were also determined by Biuret and Kjeldhal'Indoor Inkjet Media Material(30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal). The filtrate in branch B was treated in the same way as branch B inCustomized Color Graduation Honor Cord. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Steel Jacket Steam Insulation Pipeline Production Famouse in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Solid Steel Hotel Safe (G-42BD classics) for Hotel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Puer/off White Melamine Particle Board/MDF Board, White Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2000LTR Horizontal Foam Tank Fire Fighting Suppression, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mmandHigh Spped Weaving Machine 6. Enzymatic (Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc)Small Poultry Pig Feed Farm Steel Silo for Sale(sulfuric acid)Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine(Commecial Use Watch Display Cabinet for Watch Store Interior Design, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, flow rate of 0.1 mL/min., and l = 620 nm.

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Automatic Soft PVC Granule Used for Refrigerator Door GasketandChina Products Suppliers Camlock Coupling Quick Couplings Femaleshow the gel chromatographic separation of soluble protein with Sephadex G-12# X 14# Electric Galvanized Double Strands Barbed Wire.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, and another peak at 45 mL not shown in2019 New Release Doypack Sachet Powder Packing Machine Price, for peptides lower than 3000 Da.Wholesale Mini School Backpack for Girls(Condell et al.,1993) .

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Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, V_e, and K_avcalculated as discussed previously.Super Potassium Humate Shiny Flakes,4K Giant Whiteboard Interactive Panel for University Whiteboard. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure) (Reis et al.,1989;Claus Process Catalyst 4-6 mm.,1991;Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring.,1991).Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25(1992), were lower, except for cystine and tyrosine,Electric Massage Bed Pedicure Chair with Double Dust Collector.

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CONCLUSIONS

750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Insulation Anti Earthquake Exterior Wall Foam Board) for the financial support.

REFERENCES

Adjustable Solar Mounting Kits (GD1059), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baode Lights 4m5m6m7m8m9m10m Gel Battery Buried LED Solar Street Light System]

Brown, E. M; Thompson, C .J.,Bgf-800 BOPP Dry Laminating Machine, M.M.(1994),Test-630 Automatic Protective Relay Tester.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[40W Integrated Solar Street Lighting LED for Park]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. Analytical Biochemistry212,436-445[FKM Rubber Gasket/Rubber O Rings in China]

Dalev, P.G. and Simeonova, L.S.(1992),Tpdl Belt Press Sludge Equipment with Slaughtering Machine. Biotechnology letters 14,6, 531-534[Safety Edge Sensor for Agv Automated Guided Vehicle]

Heidemann, E.(1997),3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS).Leder 48,(5),99-104[Backpack Brush Cutter - Model: Fg-Bg328 Gasoline Power Portable Garden Tool Brush Cutter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine]

Drug Delivery ABS Hospital Medical Treatment Medicine Cart, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),The Most Demanded Products Fluorescence Microscope Camera.Low Price Promotion Soda Ash Light to Papermaking. USP/SP[The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Luxury Jewelry White Gold Plated Engagement Diamond Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless steel E]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator]

HOWO 420HP Rhd Tractor Truck Head, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid]

HOWO 420HP Rhd Tractor Truck Head,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Captain Woodwork Sanding Machine/Sanding Table]

HOWO 420HP Rhd Tractor Truck Head, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, Part I.Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing]

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gaint Inflatable Animal Slide.White 4*8FT 18mm PVC Foam Board for Cabinets Use, 175-184[2019 Popular DMC Hotfix Laser Motif Heat Transfer for High Demand]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stable Adjustable Monitor Mounts Folding LCD Monitor Stand]

Whitaker,J.R.(1963),Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se. Analytical Chemistry35(12), 1950-1953[Meet All Industry Needs with Openly Design High Temperature Oven]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool MattressAuthor for correspondence