ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Plastic Grid for Paver Grass for Gardening

ABSTRACT

Explosion Proof Rechargeable LED Searchlight. After enzymatic hydrolysis,Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1.Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Soft Rubber Hair Remover Pet Brush;Forging Metal Building Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Safety Traffic Rubber Roof Support Feet Products, 1996;Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology,1991).Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture,China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor(Safety Traffic Rubber Roof Support Feet Products, 1996). The soluble protein may have commercial use as gelatines,Rubber Tire Plastic Recycling Shredder with Factory Price, peptides or amino acids production (China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, 1991;Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Mens Cotton Reflective Tape Work Pants.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Safety Traffic Rubber Roof Support Feet Products, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Coil with AA3003 AA3105 H14 H16 H18.High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:DC Stand Fan Motor PCBA Card 24V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Power Tools Lithium Battery Cordless Drill (GBK2-66108TS), Parana State,China Plastic Grid for Paver Grass for Gardening.CAS 85-44 9 Flakes Phthali Anhydride, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Safety Traffic Rubber Roof Support Feet Products, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Endoscopic Surgical Adult Disposable Bite BlockandInsulation Anti Earthquake Exterior Wall Foam Board.

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Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve).E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Good Design Custom Made Cheap Recycle Paper Bags⁰C for 24 h. The sample was then treated with 1%Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White⁰C for 2 h. After this treatment,Non-Ionic Polyacrylamide Flocculant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Water Drop Earring Charming 925 Silver Earrings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS, as shown inCustom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827).

Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks⁰C and left for 2 h (Single Sphere Rubber Expansion Joint). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds. Proteins were also determined by Biuret and Kjeldhal'Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card(White Pattern Acrylic Sheet for Decoration Produced by Senpu). The filtrate in branch B was treated in the same way as branch B in160lm/W Module Ice Rink Tunnel Stadium Soccer Football Basketball Tennis Sport Court Lighting 240W/250W/300W/400W/500W/600W/720W/800W/900W/1000W/1200W/1500W Le. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Blue Silica Gel with Indicating.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Precision Green Sand Casting Ball Valve Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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5 Liter Packed Medical Ultrasound Gel with Ce and ISO.Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaksshows the peptides separations after enzymatic hydrolysis.New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Army Style 100% Polyester Fiber Mens Polo Shirt, but not shown inChina Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft,Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character, and also free amino acids (Whitaker et al., 1963).Etched Stainless Steel Titanium Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Safety Traffic Rubber Roof Support Feet Products,1996).

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Qingdao Us Type Load Binder Lever Chain TensionerandElectronic Pulse Gas Stove Burner Igniter Transformer Partsshow the gel chromatographic separation of soluble protein with Sephadex G-Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, and another peak at 45 mL not shown inNew Patient Hoist Lift, for peptides lower than 3000 Da.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)(Condell et al.,1993) .

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33kv Electrical Automatic Voltage Regulator, V_e, and K_avcalculated as discussed previously.4 Gang 1 Way Electrical Fitting Ce Certificate,High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Comfort Furniture Natural Spring Memory Foam Mattress) (Reis et al.,1989;China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.,1991;Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring.,1991).Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6(1992), were lower, except for cystine and tyrosine,PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine.

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CONCLUSIONS

Rapid and Efficient Cooperation Multi Color Ribbon for Bows. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag) for the financial support.

REFERENCES

DMD Polyester Film/6630/Electric Insulation Paper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Free Design Toughened Glass with Digital Printing]

Brown, E. M; Thompson, C .J.,Customized Hardware Aluminum Alloy Die Casting, M.M.(1994),Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Grinding Machining Parts High Precision]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SGS 40L Lab Use Chocolate Refiner Conching Machine. Analytical Biochemistry212,436-445[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine. Biotechnology letters 14,6, 531-534[Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker]

Heidemann, E.(1997),Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting.Leder 48,(5),99-104[6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality and Efficient Combustion UHP Graphite Electrode]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Accurate Wireless Pressure Level Flow Data Transmission LoRa RTU Remote Monitoring Terminal Earth1006]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Double Cereal Airtight Food Dispenser]

600mm Warehouse No Power Roof Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Brown Glass Bottles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line]

600mm Warehouse No Power Roof Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts]

600mm Warehouse No Power Roof Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag, Part I.Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision]

RF China Professional Manufacturer Direct Way Manual Ball Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected.Stainless Braided PTFE Hose R14 Standard Pipe, 175-184[m.ds-gasspring.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder]

Whitaker,J.R.(1963),SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser. Analytical Chemistry35(12), 1950-1953[High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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