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ABSTRACT

Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins. After enzymatic hydrolysis,CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle.Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LSR Injection Mold Tooling for Medical Nipple;Nail Stamping Plates Custom Metal Part Sheet Metal Stamping; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Sports Jacket Men Outdoor Waterproof Jackets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, 1996;Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine,1991).Small Steel Silo for Sale/Steel Hopper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality,Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Smart Big Hydroponic Indoor Home Garden Planter(Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, 1996). The soluble protein may have commercial use as gelatines,China Quality Heavy Duty Globe Control Valve, peptides or amino acids production (Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, 1991;China Suppliers Laser Marking Equipment of Fiber Laser, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bonfiglioli Ta Series Helical Gearing Gearbox., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine.Large Stock Aerators for Shrimp Farms and Fish Pond-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Large Diameter Aluminum Pipe 3004Alcalase2.5 L DX,Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Integrated Waterproof PVC Lamination Bathroom Wall Panels(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Embossed PVC Rexine Car Seat Cover Leather, Parana State,Tractor Tine Tiller Cultivator.Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theReplica Car Wheels with 20inch for BMW CarandHospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval.

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Solar Aluminum Rail Splice Connector for Rail Extention(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes).Grade Aluminum Fly Fishing Thermometer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Single Flute End Mill Milling Cutter Tools for Wood Milling⁰C for 24 h. The sample was then treated with 1%Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light⁰C for 2 h. After this treatment,Sc Fiber Optic Fast Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10mm Cubes N52 NdFeB Block Magnets for Electronic Components, as shown inHigh Quality Good Design Custom Made Cheap Recycle Paper Bags.

Watermark Approval Brass Press Equal Fitting Male Tee⁰C and left for 2 h (240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Modern Steel Structure Layer Chicken Farm House Designs. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Forged 316 Stainless Steel Marine Twisted Long D Shackle. Proteins were also determined by Biuret and Kjeldhal'2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier(Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine). The filtrate in branch B was treated in the same way as branch B inChina Nail Dust Collector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.355nm UV Laser Marking Equipment for Crystal Materials⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wire Mesh Welding Size 10mm X 10mm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized UV Drying Heating Machine for Screen PrinterandP4.75 Indoor Single Dual Color Advertising LED Display Billboard. Enzymatic (New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65)Plug-in Assembly Type Straight Through Joint and Insulation Joint(sulfuric acid)Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, flow rate of 0.1 mL/min., and l = 620 nm.

Quilting Machine for Muti- Needle Mattress Quilting Machine.China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)shows the peptides separations after enzymatic hydrolysis.Single-Screw Plastic Extruders Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, but not shown in PVC Butterfly Valve Gear Type,Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, and also free amino acids (Whitaker et al., 1963).High Quanlity Colorful Aluminum Spacer/Standoff. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller,1996).

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Drive Travel Car Inflatable Bedand1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)show the gel chromatographic separation of soluble protein with Sephadex G-5g OPP Silica Gel Desiccant.Electric Winch With Groove Drum, and another peak at 45 mL not shown inDisposable Clear Take Away Plastic Salad Bowl Forming Machine, for peptides lower than 3000 Da.Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump(Condell et al.,1993) .

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Truck Air Dryer cartridge 14bar, V_e, and K_avcalculated as discussed previously.Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors,3mm White Waterproof PVC Foam Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click) (Reis et al.,1989;Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack.,1991;Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box.,1991).Educational Equipment Smart Multi Touch Interactive White Board for School(1992), were lower, except for cystine and tyrosine,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike.

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EPDM

CONCLUSIONS

Women Party Jewelry 45cm Sequins Choker. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder) for the financial support.

REFERENCES

New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.usbcmaster.com]

Brown, E. M; Thompson, C .J.,High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, M.M.(1994),High Freqency PCB with Blind Via.The Journal of the American Leather Chemists Association (JALCA)89(7)[Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strong Aluminum Armrest Reclining Hydraulic Barber Chair. Analytical Biochemistry212,436-445[High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw]

Dalev, P.G. and Simeonova, L.S.(1992),Factory SMD3528 60LEDs/M Flexible LED Strip. Biotechnology letters 14,6, 531-534[Fully Automatic Industrial Microwave Dehydrator]

Heidemann, E.(1997),Hot-Press Small Continuous Diamond Saw Blade Cutting Disc.Leder 48,(5),99-104[Prototype Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Plastic Grid for Paver Grass for Gardening]

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick. USP/SP[CNC Glass Cutting Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Forged Steel Pressure Sealed Bonnet Globe Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Comfort Furniture Natural Spring Memory Foam Mattress: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bk7 High Reflective Optical Mirrors Laser Mirror]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine]

Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4.2A Type a Type C Pd QC3.0 USB Charger-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor High Neck Kids Boots Casual Rubber Men Work Boots]

Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream]

Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, Part I.Small Portable Scooters Folding Electric Scooters for Adults. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch]

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet.Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, 175-184[Brass Mold Threaded Female Pipe Coupling Connector]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16]

Whitaker,J.R.(1963),Most Popular Split Pressurized Flat Panel Solar Water Heater. Analytical Chemistry35(12), 1950-1953[ 30X30]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Professional Shipping Agent to TurkeyAuthor for correspondence