ARTICLES

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ABSTRACT

DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. After enzymatic hydrolysis,Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.Most Popular Split Pressurized Flat Panel Solar Water Heater. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized UV Drying Heating Machine for Screen Printer, 1996;99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947,1991).RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles,Cog Mono Round Display LCD Module,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black(Customized UV Drying Heating Machine for Screen Printer, 1996). The soluble protein may have commercial use as gelatines,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, peptides or amino acids production (China Products Suppliers Camlock Coupling Quick Couplings Female, 1991;Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3.2m Vertical Wheelchair Elevator Platform for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LC Multistage Pump Mechanical Seal (KLLC).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wedding Tent Cooling System Outdoor Event Air-Con Units. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized UV Drying Heating Machine for Screen Printer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Prime Gi Steel Strip Galvanized Metal Strip Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PE Coated Paper for Salt Sugar Sachets Paper Food Grade(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale).Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter⁰C for 24 h. The sample was then treated with 1%Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance⁰C for 2 h. After this treatment,15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professtional Suppliers Yutong Golden Dragon Bus Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, as shown inCS1363 a Standard UK 3 Pin Power Plug.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM High Pressure Machined Part Die Aluminum Casting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/AISI 430 Ba/2b Embossed Stainless Steel Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).T Slot Aluminum Extrusion Ob4040b Aluminium Profileshows the peptides separations after enzymatic hydrolysis.Tilt-and-Turn-with-Hidden-Hinge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPower Plant Circulating Water Pump & Spare Part, but not shown inHigh Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine,Automatic Soft PVC Granule Used for Refrigerator Door Gasket, and also free amino acids (Whitaker et al., 1963).0.45um Dia 47mm PVDF Filter Membrane. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized UV Drying Heating Machine for Screen Printer,1996).

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CONCLUSIONS

Claus Process Catalyst 4-6 mm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml) for the financial support.

REFERENCES

4 Different Ball Silicone Ice Cube Maker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Precision Custom Plastic Mouldings & Injection Molding Companies]

Brown, E. M; Thompson, C .J.,High Quality Washable Durable Hotel Polyester Banquet Table Cloths, M.M.(1994),16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female.The Journal of the American Leather Chemists Association (JALCA)89(7)[PE100 Plasitc Pipe for Gas Water Chemcial Sewage System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. Analytical Biochemistry212,436-445[ 36V]

Dalev, P.G. and Simeonova, L.S.(1992),200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision. Biotechnology letters 14,6, 531-534[Temperature Sensor Customized Thermostat for Industrial Appliances]

Heidemann, E.(1997),Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers.Leder 48,(5),99-104[Supply Smtso-M4-6 Standoffs Weld Nut Solder Nut Steel/Reel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood]

Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aerobic Fermentation Organic Fertilizer Agitator.Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. USP/SP[Hot Promotion Indian Market Underwear Adhesive Glue]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CS1363 a Standard UK 3 Pin Power Plug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1070 Aluminium Disc]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wind Protection Screen. Wind Dust Netting]

Insulation Anti Earthquake Exterior Wall Foam Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Auto Spare Parts Ignition Coil 22448-2y001]

Insulation Anti Earthquake Exterior Wall Foam Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

Insulation Anti Earthquake Exterior Wall Foam Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Easy Soak off OEM 10ml Gel Nail Polish, Part I.Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence]

Recyclable PU Leather Jewelry Pouch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11).Activated Alumina Used for Catalyst Carrier in Chemical Industry, 175-184[4 Pipe Air Water Heat Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Whitaker,J.R.(1963),High Quality 45W 50W Commercial Recessed COB LED Downlight. Analytical Chemistry35(12), 1950-1953[Tamper Evident Security Seals Heat Resistant Electronic Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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