ARTICLES

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ABSTRACT

Breathable Surgical Paper Tape/ Micropore Medical Tape. After enzymatic hydrolysis,Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow.Crane Hook Qtz250 (7032) with 70m Jib Length. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Online High Sensitivity Checkweigher with Pusher Bar, 1996;Piggery Farm Pig/Sow Gestation Equipment From China Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lw250*700 Spiral Discharge Decanter Separator for Water Treatment,1991).Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Children Gift Outdoor Wooden Rocking Horse Toy,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass(Online High Sensitivity Checkweigher with Pusher Bar, 1996). The soluble protein may have commercial use as gelatines,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, peptides or amino acids production (ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, 1991;China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor High Neck Kids Boots Casual Rubber Men Work Boots.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Online High Sensitivity Checkweigher with Pusher Bar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector.2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Grinding Machining Parts High Precision(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Cheap Newest 70*77cm XPE Foam 3D Wallpaper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve) for the financial support.

REFERENCES

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.kseidon.com]

Brown, E. M; Thompson, C .J.,Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, M.M.(1994),Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 40FT]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). Analytical Biochemistry212,436-445[Retractable Multi Core Cable Reel]

Dalev, P.G. and Simeonova, L.S.(1992),Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. Biotechnology letters 14,6, 531-534[LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nickel 200 Floating Ball Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rapid and Efficient Cooperation Multi Color Ribbon for Bows]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Series 10 Ton Mdg (L) Type Main Single Girder Gantry Crane Price with Good Quality]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps]

Industrial Mens Cotton Reflective Tape Work Pants, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm]

Industrial Mens Cotton Reflective Tape Work Pants,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine]

Industrial Mens Cotton Reflective Tape Work Pants, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Coil with AA3003 AA3105 H14 H16 H18, Part I.High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Inflatable Cheering Stick for Promotional Gift]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gold Inlaid 24K Gold / Flowers]

Whitaker,J.R.(1963),Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag. Analytical Chemistry35(12), 1950-1953[Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic GlassAuthor for correspondence