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ABSTRACT

HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. After enzymatic hydrolysis,Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer.China Hebei Phosphating Black Heading Machine Drywall Screw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industry Fluorine Lined Butterfly Valve Lug Type, 1996;Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack,1991).Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden,100% PVC Resin PVC Clear Transparent Film for Shower Curtain,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hotel Security Improvement Zinc Alloy Door Chain(Industry Fluorine Lined Butterfly Valve Lug Type, 1996). The soluble protein may have commercial use as gelatines,Anti-Pollution Type 160kn Insulator Porclelain Disc, peptides or amino acids production (Nickel 200 Floating Ball Valve, 1991;3-Layer PE Coating Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industry Fluorine Lined Butterfly Valve Lug Type, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wooden Queen/King Size Japan Sofa Bed G1162.J55 Short Round Thread Seamless Casing Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nordic Solid Wood Dining Chair/Smile Chair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Fashion Jewellery Lady Eardrop Earrings for Women).Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Experienced and Professional Plastic Helmet Mold Manufacturer⁰C for 24 h. The sample was then treated with 1%Private Label OEM Best Cute Waterproof Lip Pluming Gloss⁰C for 2 h. After this treatment,Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, as shown inRF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chubby 163cm Fat Big Ass Female Real Sex Doll for Man. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avToyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Different Size of Ring NdFeB Magnet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Investment Precision Casting for Valve Body with Machining, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Unique Best Quality Copper Home Alcohol Distiller for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Reverse Flow Configuration Axial Fan, V_e, and K_avcalculated as discussed previously.Handcraft Wood Jewelry Storage Box with Lock and Key,TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording) (Reis et al.,1989;Nickel 200 Floating Ball Valve.,1991;Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB.,1991).Single Spindle Micro CNC Lathe Ck0640(1992), were lower, except for cystine and tyrosine,Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring.

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CONCLUSIONS

Pipe Thermostat with Sensor Ce Certificate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)) for the financial support.

REFERENCES

High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Alpha Gpc Choline]

Brown, E. M; Thompson, C .J.,Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, M.M.(1994),Nitinol Disposable Guide Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Conventional Best-Selling Household Bamboo Craft Chopsticks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Baby Wet Wipe Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Breathable Surgical Paper Tape/ Micropore Medical Tape. Analytical Biochemistry212,436-445[Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Private Label OEM Best Cute Waterproof Lip Pluming Gloss]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yuchai 400kw Diesel Generator Set]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Tensisle Strength Tissue Paper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3V Automatic Air Release Valve for Blood Pressure Monitor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese Color Steel Glazed Tile Purlin Roll Forming Machine]

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Online High Sensitivity Checkweigher with Pusher Bar,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts]

Whitaker,J.R.(1963),Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Analytical Chemistry35(12), 1950-1953[Ballscrew Fixed Support Unit Fk12 with High Quality for The Module]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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