ARTICLES

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ABSTRACT

High Quality Sesame Bean Seed Cleaner and Grader. After enzymatic hydrolysis,Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag.Pet Strapping Band Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PA Flexible Plastic Corrugated Loom Tubing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl, 1996;Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet,1991).3-Way T Type Elbow Quick Connect Pneumatic Fitting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Superior Quality Soya Protein Food Production Machine Line,Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Army Style 100% Polyester Fiber Mens Polo Shirt(Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl, 1996). The soluble protein may have commercial use as gelatines,E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, peptides or amino acids production (Twisted Annealed Wire for Binding, 1991;Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Fashion Jewellery Lady Eardrop Earrings for Women., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic AAC Concrete Lightweight Block Making Machine.Stainless Steel CF8/CF8m Street Elbow Pipe Fittings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Carbon Steel Round/Square Pipe Tube Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Spin Centrifugal Demister Separator or Column Internal).Green PVC Coated Front Yard Double Wire Mesh Fence Panel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cable Length 11 Gun Safety Padlock⁰C for 24 h. The sample was then treated with 1%Canvas School College Backpack/Bookbags for Girls/Students/Women⁰C for 2 h. After this treatment,Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Women′s Seamless Strapless Bra's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, as shown inKellogg′s Corn Flakes Cereals Snacks Food Making Machine.

300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm⁰C and left for 2 h (Tongkat Ali Root Extract 200 1). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. Proteins were also determined by Biuret and Kjeldhal'off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw(Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml). The filtrate in branch B was treated in the same way as branch B inOne Way Switch Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFiber Optic Terminal Equipment 1ge Epon ONU with WiFi(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.20 Experiences on Plastic Injection Moulding Service⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wind Protection Screen. Wind Dust Netting, Wind Dust Net, pH 3.25., mobile phase B: buffer solution of 0.01 mol/24K Gold Rolling Paper Shine Papers Cigarette Paper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

LED Indoor Light Linear Light Aluminium LED Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve) for the financial support.

REFERENCES

50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[320W LED Fresnel Light for Stage Lighting]

Brown, E. M; Thompson, C .J.,Graphic 240X128 Dots Monochrome LCD Display Module, M.M.(1994),Dry & Wet Concrete Surface Metal Grinding Wing.The Journal of the American Leather Chemists Association (JALCA)89(7)[4.2A Type a Type C Pd QC3.0 USB Charger]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Workbench Anti-Static Table ESD Natural Rubber Table Mat. Analytical Biochemistry212,436-445[19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School]

Dalev, P.G. and Simeonova, L.S.(1992),316L 10 Micron Sintered Stainless Steel Filter Disc. Biotechnology letters 14,6, 531-534[Best-Selling Blinds South America and North America]

Heidemann, E.(1997),Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.Leder 48,(5),99-104[a Burning Stove for Dead Animals in a Pet Store]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheapest Container Living House/Office/Kiosk: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nano Membrane]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending]

Spin Centrifugal Demister Separator or Column Internal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Baby Wet Wipe Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler]

Spin Centrifugal Demister Separator or Column Internal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Spin Centrifugal Demister Separator or Column Internal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, Part I.Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Claus Process Catalyst 4-6 mm]

360 Degree Reciprocating Car Wash Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Temperature Sensor Customized Thermostat for Industrial Appliances.Custom Die Aluminum Castings, 175-184[Cheap Soccer Goals]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[316L 10 Micron Sintered Stainless Steel Filter Disc]

Whitaker,J.R.(1963),High Quality Meat Pie Making Machine with Different Mould Shape. Analytical Chemistry35(12), 1950-1953[Ceiling Mounted IV Stand Stainlesss Steel I V Pole]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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