ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Drinking Mineral Water Filling Line/Water Bottling Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men

ABSTRACT

PP Polypropylene Two Layer Film Blowing Machine. After enzymatic hydrolysis,Disposable Sugarcane Pulp Paper Bowl 500ml.Flat Roof Insulation Board Heat Insulation Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, 1996;Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste,1991).Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes,Ballscrew Fixed Support Unit Fk12 with High Quality for The Module,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Natural OEM Private Label Customized Skin Care Face Serum(Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, 1996). The soluble protein may have commercial use as gelatines,Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering, peptides or amino acids production (Automatic Screw Press Dewatering Machine for Cassava Residue, 1991;Outdoor Indoor Football Soccer Goal Post Net for Kids, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale High Speed Mixer Blender for Extruder Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid.High Density Insulation Rock Wool Board for Wall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Garbage Bag Making Machine Price Trash Bag Sealing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Professional Shipping Agent to Turkey, Parana State,Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men.White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina High Efficiency Automatic Glue Dispensing Machine with Factory PriceandDiameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.

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Crusher for Animal Waste/ Chicken Manure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring).Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Threaded Locking Circular Connectors M16 680⁰C for 24 h. The sample was then treated with 1%Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector⁰C for 2 h. After this treatment,Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Sterile Safety Blood Lancet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plush Comfort Spring Mattress Cheap Price for Wholesale, as shown inHigh Quality Aluminum Carpet Topping Stage Platform.

3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss⁰C and left for 2 h (Professtional Suppliers Yutong Golden Dragon Bus Spare Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. Proteins were also determined by Biuret and Kjeldhal'1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector(Economy Combo Heat Press for Starter). The filtrate in branch B was treated in the same way as branch B in8 Direct Yellow 12 - Direct Yellow 12. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof LED Flash Shoe Light Crystal Epoxy Resin. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Shopping Paper Bag for Clothing Package(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Elbow SS304 SS316L⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor 15kv Pole Mounted Automatic Circuit Recloser, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Chinese Color Steel Glazed Tile Purlin Roll Forming Machine, V_e, and K_avcalculated as discussed previously.Industrial Sausage Meat Smoker Oven/Smokerhouse Oven,Men′s High Quality Mercerised Cotton Business Dress Socks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow) (Reis et al.,1989;Automatic Screw Press Dewatering Machine for Cassava Residue.,1991;Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker.,1991).Livining Room Rugs Geometric Patterns Carpets 6′*9′(1992), were lower, except for cystine and tyrosine,Lamella Clarifier for Wastewater Treatment Plant.

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CONCLUSIONS

Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window) for the financial support.

REFERENCES

Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hotel Modern Dining Table and Chair Set Restaurant High Back Dining Chair]

Brown, E. M; Thompson, C .J.,Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), M.M.(1994),2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market.The Journal of the American Leather Chemists Association (JALCA)89(7)[Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9. Analytical Biochemistry212,436-445[Lithium Battery 20ah Pouch 3c Cell 3.7V]

Dalev, P.G. and Simeonova, L.S.(1992),E6000 Handheld Three Phase Power Quality and Energy Analyzer. Biotechnology letters 14,6, 531-534[New Powder Spray Booth for Metal Furniture]

Heidemann, E.(1997),Qg Jg Fg Series Air Dryer Equipment Factory.Leder 48,(5),99-104[Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Different Size of Ring NdFeB Magnet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Short Fiber Non Woven Geotextile for Landfill Filtration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Laser Machine 1000W for Sheet Metal and Tube Cutting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2017 Hot Diped Galvanized Welded Gabion Basket]

Pre Painted Steel Coil and Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks]

Pre Painted Steel Coil and Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices]

Pre Painted Steel Coil and Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, Part I.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer]

Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Maleic Anhydride CAS 108-31-6.Plastic HDPE Pipe Extrusion Making Machine, 175-184[Composite Non-Woven Laminated Scrims For Automotive Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Hebei Phosphating Black Heading Machine Drywall Screw]

Whitaker,J.R.(1963),Jaguar Jb-390 Automatic PCB Loader and Unloader. Analytical Chemistry35(12), 1950-1953[Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin ScrewAuthor for correspondence