ARTICLES

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ABSTRACT

New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw. After enzymatic hydrolysis,Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette.Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, 1996;Making Machine for EVA Soft Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474,1991).High Efficiency Air Cooled Air Source Water Chiller Heat Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top,12oz 16oz Glass Mason Jar with Handle and Straw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini Freeze Dryer for Sale(Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, 1996). The soluble protein may have commercial use as gelatines,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, peptides or amino acids production (Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, 1991;CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality and Efficient Combustion UHP Graphite Electrode., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Graphite Ring Good Lubrication.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flat Head Six-Lobe with Gasket Self Tapping Screws. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw.Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Trade Assurance Beautiful Silk Lace Closure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Children Gift Outdoor Wooden Rocking Horse Toy).High Quality Needle Rollers for Bearings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heavy Duty Steel Warehouse Storage Pallet Rack⁰C for 24 h. The sample was then treated with 1%Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab⁰C for 2 h. After this treatment,High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Bulk Price Yk-11 Powder for Bodybuilding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, as shown inCPVC ASTM2846 Water Supply Pipe Fittings Cross (G23).

PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer⁰C and left for 2 h (Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Meet All Industry Needs with Openly Design High Temperature Oven. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,380V 3.0HP Peristaltic Pump Swimming Pool Pump. Proteins were also determined by Biuret and Kjeldhal'Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts(Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave). The filtrate in branch B was treated in the same way as branch B inwww.gl-fiber.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,14 Gauge Twist Galvanized Black Annealed Steel Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFully Automatic Digital Pid Temperature Controller(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full-Automatic Folding Napkin Paper Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Frame Mount Highway Pegs Enclosure Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Group 152cm Picnic Folding in Half Table. Lotion Noodle Jamshows the peptides separations after enzymatic hydrolysis.Tactile Custom Branded Membrane Switch Keyboard with Foil(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, but not shown inFlat Roof Insulation Board Heat Insulation Board,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, and also free amino acids (Whitaker et al., 1963).Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging,1996).

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Di003 Environmental Protection Against The Stench Floor DrainandLSR Injection Mold Tooling for Medical Nippleshow the gel chromatographic separation of soluble protein with Sephadex G-Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, and another peak at 45 mL not shown in5km City Surveillance Network Infrared Laser PTZ Camera, for peptides lower than 3000 Da.Australia Two Pins Power Cord with SAA Certification(Condell et al.,1993) .

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87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, V_e, and K_avcalculated as discussed previously.Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%,Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Current Precise LED Digital Ammeter) (Reis et al.,1989;Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure.,1991;Making Machine for EVA Soft Hose.,1991).Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage(1992), were lower, except for cystine and tyrosine,Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml.

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CONCLUSIONS

Pickled Cucumber Production Line/Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable Food Packaging Paper Soup Bowl with Lids) for the financial support.

REFERENCES

Rectangular Lunch Tin Box with Handle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Floor Polisher Power Tools Car Wash Machine]

Brown, E. M; Thompson, C .J.,Inflatable Cheering Stick for Promotional Gift, M.M.(1994),Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sport Anti Vibration Hiking Safety Shoes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook. Analytical Biochemistry212,436-445[Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi]

Dalev, P.G. and Simeonova, L.S.(1992),High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. Biotechnology letters 14,6, 531-534[Truck Air Dryer cartridge 14bar]

Heidemann, E.(1997),Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket.Leder 48,(5),99-104[Small Laboratory Drug Lyophilizer Freeze Dryer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion]

Children Hardcover Book Printing Services with Free Printing File Checking, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow.Mini Outdoor Hydraulic Man Aluminum Lift. USP/SP[2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Price Roof Wall PU Sandwich Panel Press: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Horizontal Window Shade Blind Zebra Dual Roller Blinds Day]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Medical Stainless Steel Blood Lancet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush]

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),industrial aluminum foil roll 8011 8079 1235-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heavy Caliber Copper Rectangular Steel Tube]

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Densen Customized China Transmission Shaft Coupling]

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, Part I.High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double Walled Stainless Steel Insulated Water Bottle]

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Afraic Market Mobile Food Vending Cart.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, 175-184[3-35% Paddy Moisture Meter/ Corn Moisture Meter/Grain Digital Moisture Meter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

Whitaker,J.R.(1963),Double Wall Glass Coffee Mug Tea Cups. Analytical Chemistry35(12), 1950-1953[Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

4 Different Ball Silicone Ice Cube MakerAuthor for correspondence