ARTICLES

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ABSTRACT

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. After enzymatic hydrolysis,2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price.Tractor Tine Tiller Cultivator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins;CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LSR Injection Mold Tooling for Medical Nipple, 1996;Nail Stamping Plates Custom Metal Part Sheet Metal Stamping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Sports Jacket Men Outdoor Waterproof Jackets,1991).Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Steel Silo for Sale/Steel Hopper(LSR Injection Mold Tooling for Medical Nipple, 1996). The soluble protein may have commercial use as gelatines,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, peptides or amino acids production (Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights, 1991;Smart Big Hydroponic Indoor Home Garden Planter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Quality Heavy Duty Globe Control Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Suppliers Laser Marking Equipment of Fiber Laser. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LSR Injection Mold Tooling for Medical Nipple, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bonfiglioli Ta Series Helical Gearing Gearbox.Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, Parana State,Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices.OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (LSR Injection Mold Tooling for Medical Nipple, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRight Angle Worm Gearbox for Food and Beverage and Packaging MachinesandCentral Tube GYXTW 4 Core Singlemode Fiber Optic Cable.

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Integrated Waterproof PVC Lamination Bathroom Wall Panels(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting).Embossed PVC Rexine Car Seat Cover Leather(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves⁰C for 24 h. The sample was then treated with 1%Solar Aluminum Rail Splice Connector for Rail Extention⁰C for 2 h. After this treatment,Grade Aluminum Fly Fishing Thermometer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Single Flute End Mill Milling Cutter Tools for Wood Milling's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, as shown inCNC Aluminum Alloy Metal Stamping Processing Services.

Sc Fiber Optic Fast Connector⁰C and left for 2 h (Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10mm Cubes N52 NdFeB Block Magnets for Electronic Components. Proteins were also determined by Biuret and Kjeldhal'Watermark Approval Brass Press Equal Fitting Male Tee(14 Gauge Twist Galvanized Black Annealed Steel Wire). The filtrate in branch B was treated in the same way as branch B inm.agapassworld.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Modern Steel Structure Layer Chicken Farm House Designs.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Forged 316 Stainless Steel Marine Twisted Long D Shackle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aerobic Fermentation Organic Fertilizer Agitatorand355nm UV Laser Marking Equipment for Crystal Materials. Enzymatic (Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB)Wire Mesh Welding Size 10mm X 10mm(sulfuric acid)P4.75 Indoor Single Dual Color Advertising LED Display Billboard(LSR Injection Mold Tooling for Medical Nipple, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, flow rate of 0.1 mL/min., and l = 620 nm.

Plug-in Assembly Type Straight Through Joint and Insulation Joint.High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40shows the peptides separations after enzymatic hydrolysis.Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, but not shown inHot Sale AlNiCo Cow Stomach Magnets China Manufacturer,High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, and also free amino acids (Whitaker et al., 1963).Quilting Machine for Muti- Needle Mattress Quilting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LSR Injection Mold Tooling for Medical Nipple,1996).

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Other

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5g OPP Silica Gel Desiccant, V_e, and K_avcalculated as discussed previously.Electric Winch With Groove Drum,Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden) (Reis et al.,1989;Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights.,1991;Nail Stamping Plates Custom Metal Part Sheet Metal Stamping.,1991).Truck Air Dryer cartridge 14bar(1992), were lower, except for cystine and tyrosine,Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors.

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CONCLUSIONS

3mm White Waterproof PVC Foam Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.) for the financial support.

REFERENCES

Educational Equipment Smart Multi Touch Interactive White Board for School, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Quality Porcelain Wide Rim Round Deep Plate]

Brown, E. M; Thompson, C .J.,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, M.M.(1994),Women Party Jewelry 45cm Sequins Choker.The Journal of the American Leather Chemists Association (JALCA)89(7)[24K Gold Rolling Paper Shine Papers Cigarette Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ferrous Sulphate Monohydrate 30%Min Factory Wholesale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. Analytical Biochemistry212,436-445[4K Giant Whiteboard Interactive Panel for University Whiteboard]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. Biotechnology letters 14,6, 531-534[Mini Freeze Dryer for Sale]

Heidemann, E.(1997),High Freqency PCB with Blind Via.Leder 48,(5),99-104[LED RGB Wash Wall Lighting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bending Shrapnel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot-Press Small Continuous Diamond Saw Blade Cutting Disc: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ce Factory Price PVC Rain Boot Outdoor Fashion Adult Boot Steel Toe Cap Skid Resistant/Acid and Alkali Resistant Farm/Fish Breeding (SN5315)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Comfort Furniture Natural Spring Memory Foam Mattress, Part I.Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5 Gallon Pure Water Bottle Non-Spill Cap]

4.2A Type a Type C Pd QC3.0 USB Charger, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ferrous Sulphate Monohydrate 30%Min Factory Wholesale.Small Portable Scooters Folding Electric Scooters for Adults, 175-184[Forming Stamping Parts Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

Whitaker,J.R.(1963),Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant. Analytical Chemistry35(12), 1950-1953[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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