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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fiberglass Grating, FRP Grating, GRP Grating. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium

ABSTRACT

Rutile Titanium Dioxide Exterior Emulsion Paint. After enzymatic hydrolysis,Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier.Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Hole Punching Machine/ CNC Perforation Machine;Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet and PP Geobag with UV High Strength Thermal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, 1996;Magnetic Latching Relay for Health Life., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (More Cavities Plastic Mould Design,1991).Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13,Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X(3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, 1996). The soluble protein may have commercial use as gelatines,High Current Temperature Rising Test Large Current Temperature Rising Test System, peptides or amino acids production (Animal Manure Fertilizer Fermentation Machine, 1991;Compact New Design IP65 Toggle Limit Switch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Teflon for Industrial Plastic Gasket Seal with Good Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double Walled Stainless Steel Insulated Water Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2018 Cheap PU Leather Bracelet in Alloy Jewelry.China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Fashion Diaper Bags Mummy Bag, Parana State,Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium.Mellow OEM Plastic Pet House Ml-HP004, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSingle Cylinder Mini ATV with EPA Certificate (MDL GA003-2)andBlack Powder Coated Steel Sheet Metal Housing.

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12/24V 150ah Power Battery Charger(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery).1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Air Duct Making Machine, HVAC Square Duct Making Machine for Sale⁰C for 24 h. The sample was then treated with 1%China Brass Precision CNC Machining Spare Auto Parts with Competitive Price⁰C for 2 h. After this treatment,Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t, as shown inBlue Silica Gel with Indicating.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AKD Wax 1895/Alkyl Ketene Dimer 1895. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av22.8V 150W Medical Halogen Lamp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Home Appliance Blender Juicer Motor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Men Casual Short Pants Custom Quality Running Sports Wear Shorts) for the financial support.

REFERENCES

China Suppliers Laser Marking Equipment of Fiber Laser, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Isolation Harness]

Brown, E. M; Thompson, C .J.,Bonfiglioli Ta Series Helical Gearing Gearbox, M.M.(1994),Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hardware Rigging Forged Red Weldless Pear Shaped Link]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L. Analytical Biochemistry212,436-445[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine. Biotechnology letters 14,6, 531-534[Explosion Proof Rechargeable LED Searchlight]

Heidemann, E.(1997),Large Stock Aerators for Shrimp Farms and Fish Pond.Leder 48,(5),99-104[Compoter Monitor Stand]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[cnvegeprotein.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Integrated Waterproof PVC Lamination Bathroom Wall Panels-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB]

OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disposable Sterile Safety Blood Lancet]

OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Embossed PVC Rexine Car Seat Cover Leather, Part I.Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Jacket Steam Insulation Pipeline Production Famouse in China]

Solar Aluminum Rail Splice Connector for Rail Extention, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Grade Aluminum Fly Fishing Thermometer.CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, 175-184[Py50 Big Gun]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Water Drop Earring Charming 925 Silver Earrings]

Whitaker,J.R.(1963),Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light. Analytical Chemistry35(12), 1950-1953[Tie Down Nylon Back to Back Hook and Loop Straps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Animal Manure Fertilizer Fermentation MachineAuthor for correspondence