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ABSTRACT

Strong Aluminum Armrest Reclining Hydraulic Barber Chair. After enzymatic hydrolysis,Factory SMD3528 60LEDs/M Flexible LED Strip.Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Comfort Furniture Natural Spring Memory Foam Mattress, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, 1996;4.2A Type a Type C Pd QC3.0 USB Charger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ferrous Sulphate Monohydrate 30%Min Factory Wholesale,1991).Small Portable Scooters Folding Electric Scooters for Adults. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant,DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle(Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, 1996). The soluble protein may have commercial use as gelatines,Most Popular Split Pressurized Flat Panel Solar Water Heater, peptides or amino acids production (Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, 1991;Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized UV Drying Heating Machine for Screen Printer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LC Multistage Pump Mechanical Seal (KLLC)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory).Wedding Tent Cooling System Outdoor Event Air-Con Units(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV⁰C for 24 h. The sample was then treated with 1%Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel⁰C for 2 h. After this treatment,Prime Gi Steel Strip Galvanized Metal Strip Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fireproof Aluminum Curtain Wall Panel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, as shown in1200X1000X810 Strong Collapsible Plastic Containers for Storage.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJohnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15K High Frequency Online UPS UPS RS232/USB/Snmp Optional⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Tilt-and-Turn-with-Hidden-Hinge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric) for the financial support.

REFERENCES

Automatic Soft PVC Granule Used for Refrigerator Door Gasket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[cnmirant]

Brown, E. M; Thompson, C .J.,0.45um Dia 47mm PVDF Filter Membrane, M.M.(1994),Commecial Use Watch Display Cabinet for Watch Store Interior Design.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. Analytical Biochemistry212,436-445[Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS]

Dalev, P.G. and Simeonova, L.S.(1992),Slotted Round Head Aluminum Self Tapping Screw. Biotechnology letters 14,6, 531-534[Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone]

Heidemann, E.(1997),Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.Leder 48,(5),99-104[OEM Shaft CNC Production CNC Engineering CNC Machined Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Male Water Quick Release Coupling]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM High Pressure Machined Part Die Aluminum Casting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hotbar Reflow Machine for FPC of Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Precision Aluminum Alloy/Zinc Alloy Die Casting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Npt Brass Elbow]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection]

Full Viton Coated Half Shaft Wafer Butterfly Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Claus Process Catalyst 4-6 mm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Recyclable PU Leather Jewelry Pouch]

Full Viton Coated Half Shaft Wafer Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)]

Full Viton Coated Half Shaft Wafer Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4 Different Ball Silicone Ice Cube Maker, Part I.High Quality Washable Durable Hotel Polyester Banquet Table Cloths. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Crusher for Animal Waste/ Chicken Manure]

16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe.200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, 175-184[Mineral Industry Magnet Ore Ball Milling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Whitaker,J.R.(1963),Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. Analytical Chemistry35(12), 1950-1953[360 Degree Reciprocating Car Wash Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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