ARTICLES

Activated Alumina Used for Catalyst Carrier in Chemical Industry

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ABSTRACT

Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. After enzymatic hydrolysis,Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.High Speed Auto Corrugated Carton Box Paperboard Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Waterproof BNC Connector for Industry;Indoor Inkjet Media Material; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Jacket Steam Insulation Pipeline Production Famouse in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Solid Steel Hotel Safe (G-42BD classics) for Hotel, 1996;Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Aluminum Alloy Metal Stamping Processing Services,1991).Puer/off White Melamine Particle Board/MDF Board, White Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine,2000LTR Horizontal Foam Tank Fire Fighting Suppression,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Spped Weaving Machine 6(Solid Steel Hotel Safe (G-42BD classics) for Hotel, 1996). The soluble protein may have commercial use as gelatines,Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, peptides or amino acids production (Small Poultry Pig Feed Farm Steel Silo for Sale, 1991;Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Made in China 12 Inch Aluminum Flexible Duct Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Solid Steel Hotel Safe (G-42BD classics) for Hotel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food.Safe Delivery Effective Sarms Mk 677 for Bodybuilding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12# X 14# Electric Galvanized Double Strands Barbed Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4K Giant Whiteboard Interactive Panel for University Whiteboard, Parana State,1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS).Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Solid Steel Hotel Safe (G-42BD classics) for Hotel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUpper Limbs Machine/Spare Parts for Fitness EquipmentandCustomized Specification Mesh Water Filter SS316 Filters for Liquids Filtration.

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Electric Massage Bed Pedicure Chair with Double Dust Collector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (External Wall Material Panels/Aluminum Composite Sheet).750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Adjustable Solar Mounting Kits (GD1059)⁰C for 24 h. The sample was then treated with 1%Bgf-800 BOPP Dry Laminating Machine⁰C for 2 h. After this treatment,Test-630 Automatic Protective Relay Tester. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tpdl Belt Press Sludge Equipment with Slaughtering Machine, as shown inHeat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric.

3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)⁰C and left for 2 h (Wholesale Universal Garden Smart Flower Pot). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.The Most Demanded Products Fluorescence Microscope Camera. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Price Promotion Soda Ash Light to Papermaking. Proteins were also determined by Biuret and Kjeldhal'Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle(Fashion Design Sleeveless Fitness Wear). The filtrate in branch B was treated in the same way as branch B inLarge Diameter Fused Polished Silica Quartz Glass Pipeline Quartz Tube. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HOWO 420HP Rhd Tractor Truck Head.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDecorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Gaint Inflatable Animal Slide, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White 4*8FT 18mm PVC Foam Board for Cabinets Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Vacuum Type Single Facer Corrugator, V_e, and K_avcalculated as discussed previously.Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle) (Reis et al.,1989;Small Poultry Pig Feed Farm Steel Silo for Sale.,1991;Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c.,1991).1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve(1992), were lower, except for cystine and tyrosine,125mm Wet Diamond Pad for Polishing Marble.

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CONCLUSIONS

Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, M.M.(1994),New Design Many Styles Fancy Wine Display Rack (6532).The Journal of the American Leather Chemists Association (JALCA)89(7)[Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Checkered Plate 304 316L 410 Floor Material. Analytical Biochemistry212,436-445[Pneumatic Cut off Ball Valve with Flange Thread Weld End]

Dalev, P.G. and Simeonova, L.S.(1992),Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box. Biotechnology letters 14,6, 531-534[Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25]

Heidemann, E.(1997),Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold.Leder 48,(5),99-104[Pc Sheet For Roof]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[oil pan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3-Way T Type Elbow Quick Connect Pneumatic Fitting]

Sport Anti Vibration Hiking Safety Shoes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape]

Sport Anti Vibration Hiking Safety Shoes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Soft PVC Granule Used for Refrigerator Door Gasket]

Sport Anti Vibration Hiking Safety Shoes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, Part I.24W 400W Rainproof Switch Power Adapter for LED Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Products Suppliers Camlock Coupling Quick Couplings Female]

Industrial Threaded Plastic Connector Female Electrical Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery.Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity, 175-184[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 New Release Doypack Sachet Powder Packing Machine Price]

Whitaker,J.R.(1963),Geotextile for Airport Runways/High Way, Railway. Analytical Chemistry35(12), 1950-1953[PVC Corrugated or Smooth Surface Helix Suction Water Hose]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aws A5.1 E6013 Welding Electrode in Carbon Steel StructureAuthor for correspondence