ARTICLES

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ABSTRACT

Dust Hood Fume Extraction Systems for Robotic Laser Cutting. After enzymatic hydrolysis,IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector.Nordic Solid Wood Dining Chair/Smile Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Private Label OEM Best Cute Waterproof Lip Pluming Gloss, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display, 1996;Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine,1991).IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tent Windows Use China Made PVC Transparent Clear Mesh Tarp,13 Oz Flex Banner PVC Frontlit Banner Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller(Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display, 1996). The soluble protein may have commercial use as gelatines,3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables, peptides or amino acids production (Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, 1991;Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;10mm AISI304 Carbon Steel Balls Motor Bearing for Slide., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Different Size of Ring NdFeB Magnet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Investment Precision Casting for Valve Body with Machining. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Unique Best Quality Copper Home Alcohol Distiller for Sale.Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Auto Spare Parts Ignition Coil 22448-2y001(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5km City Surveillance Network Infrared Laser PTZ Camera).3D Good Quality Laser Engraving Machine for Souvenirs Shop(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories⁰C for 24 h. The sample was then treated with 1%3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording⁰C for 2 h. After this treatment,Reverse Flow Configuration Axial Fan. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Handcraft Wood Jewelry Storage Box with Lock and Key's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, as shown inLiving Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nitinol Disposable Guide Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBreathable Surgical Paper Tape/ Micropore Medical Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Crane Hook Qtz250 (7032) with 70m Jib Length⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China PCBA Supplier Custom Game Controller PCB) for the financial support.

REFERENCES

Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Body Slimming Suit]

Brown, E. M; Thompson, C .J.,GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, M.M.(1994),Disposable Clear Take Away Plastic Salad Bowl Forming Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Walled Stainless Steel Insulated Water Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. Analytical Biochemistry212,436-445[Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks]

Dalev, P.G. and Simeonova, L.S.(1992),Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP. Biotechnology letters 14,6, 531-534[4 Different Ball Silicone Ice Cube Maker]

Heidemann, E.(1997),Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge.Leder 48,(5),99-104[PU Leather Cover Loose Sheets Note Book]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Precision Tooling Grinding Machine Part]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1860MPa Standard BS5896 7 Wire Low Relaxation PC Steel Wire Strand]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9]

Hot Selling House Floor Cleaning 360 Spin Mop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Complete Maize Starch Wet Milling Plant with High Quality]

Hot Selling House Floor Cleaning 360 Spin Mop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customization! Bdy380CD Horizontal Directional Drilling Machine]

Hot Selling House Floor Cleaning 360 Spin Mop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Advanced Unlocking Tool for Locksmith, Part I.High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker]

Fashion Design Sleeveless Fitness Wear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DC Colorful Battery Operated Small Hand Fan for Outdoor Use.Hsc Heavy Duty 5 Ton Chain Pulley Block, 175-184[Cnc Wood Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure]

Whitaker,J.R.(1963),Outdoor Christmas Decoration Highway Street Light. Analytical Chemistry35(12), 1950-1953[2070 4th Axis CNC]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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