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ABSTRACT

Current Precise LED Digital Ammeter. After enzymatic hydrolysis,Automatic Drinking Mineral Water Filling Line/Water Bottling Line.Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PP Polypropylene Two Layer Film Blowing Machine;Disposable Sugarcane Pulp Paper Bowl 500ml; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flat Roof Insulation Board Heat Insulation Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon, 1996;Custom Female Threaded Tee T Shape Pipe Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection,1991).Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker,304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools(2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon, 1996). The soluble protein may have commercial use as gelatines,8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, peptides or amino acids production (Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, 1991;Natural OEM Private Label Customized Skin Care Face Serum, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Screw Press Dewatering Machine for Cassava Residue.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Indoor Football Soccer Goal Post Net for Kids. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear.20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale High Speed Mixer Blender for Extruder Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single Block Vertical Wire Drawing Machine for Screw and Bolt Making(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Smart Big Hydroponic Indoor Home Garden Planter).China Professional Shipping Agent to Turkey(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment⁰C for 24 h. The sample was then treated with 1%Crusher for Animal Waste/ Chicken Manure⁰C for 2 h. After this treatment,Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Threaded Locking Circular Connectors M16 680's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, as shown inOEM/ODM Professional Activated Charcoal Teeth Whitennig Powder.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof LED Flash Shoe Light Crystal Epoxy Resin⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Shopping Paper Bag for Clothing Package, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Qingdao Us Type Load Binder Lever Chain Tensioner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine.Nice Quality Coloring Printing Children Board Book Printingshows the peptides separations after enzymatic hydrolysis.White Marble, Carrara White, Bianco Carrara Marble for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, but not shown inMini Double Hole ABS Plastic CNC Machined Parts Importer,Upper Limbs Machine/Spare Parts for Fitness Equipment, and also free amino acids (Whitaker et al., 1963).Cvkr Silent Check Valve Dn350. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon,1996).

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CONCLUSIONS

Men′s High Quality Mercerised Cotton Business Dress Socks. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Garbage Bag Making Machine Price Trash Bag Sealing Machine) for the financial support.

REFERENCES

Livining Room Rugs Geometric Patterns Carpets 6′*9′, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Greenhouse House]

Brown, E. M; Thompson, C .J.,Lamella Clarifier for Wastewater Treatment Plant, M.M.(1994),Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Sheet Metal Case Parts for Industrial Computer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen. Analytical Biochemistry212,436-445[Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile]

Dalev, P.G. and Simeonova, L.S.(1992),Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S). Biotechnology letters 14,6, 531-534[Wall Mounting Metal Control Box Control Desk]

Heidemann, E.(1997),2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market.Leder 48,(5),99-104[Yg Anticorrosion Cloth Duct Tape]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Qg Jg Fg Series Air Dryer Equipment Factory: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Etched Stainless Steel Titanium Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory-Direct C7 Precision Telescoping Ball Screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Turned Aluminum Anodized Auto Part]

Motor Bearing 6014 2RS Deep Groove Ball Bearing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ml33-2mm Eddy Current Linear Position/Displacement Sensor]

Motor Bearing 6014 2RS Deep Groove Ball Bearing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

Motor Bearing 6014 2RS Deep Groove Ball Bearing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet Short Fiber Non Woven Geotextile for Landfill Filtration, Part I.Pre Painted Steel Coil and Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), 175-184[Black Rectangular Steel Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Waterproof BNC Connector for Industry]

Whitaker,J.R.(1963),Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion. Analytical Chemistry35(12), 1950-1953[Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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