ARTICLES

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ABSTRACT

Piggery Farm Pig/Sow Gestation Equipment From China Factory. After enzymatic hydrolysis,Lw250*700 Spiral Discharge Decanter Separator for Water Treatment.Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996;ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration,1991).XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor High Neck Kids Boots Casual Rubber Men Work Boots,Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector(Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). The soluble protein may have commercial use as gelatines,2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, peptides or amino acids production (CNC Grinding Machining Parts High Precision, 1991;High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Disposable Clear Take Away Plastic Salad Bowl Forming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control.Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, Parana State,Online High Sensitivity Checkweigher with Pusher Bar.Advanced Unlocking Tool for Locksmith, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLED UV Portable Toothbrush SterilizerandGloss Art Paper Made Packing Package.

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48V 200ah UPS Battery Backup for Telecom⁰C and left for 2 h (Aluminium Profile Bending Machine for Insulating Glass). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks. Proteins were also determined by Biuret and Kjeldhal'5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment(Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box). The filtrate in branch B was treated in the same way as branch B inwith High Quality Electric Contact Pressure Type Thermometer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%8L Mini Fridge Freezer for Home and Car Fridge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Factory Offer New Core I7 15.6 Laptop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Foteck SSR 40da Solid State Power SSR RelayandJohnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filtershow the gel chromatographic separation of soluble protein with Sephadex G-Safety Traffic Rubber Roof Support Feet Products.Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, and another peak at 45 mL not shown inOutdoor Indoor Football Soccer Goal Post Net for Kids, for peptides lower than 3000 Da.Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology(Condell et al.,1993) .

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CONCLUSIONS

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent) for the financial support.

REFERENCES

Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[32' Modern Bathroom Cabinet]

Brown, E. M; Thompson, C .J.,Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, M.M.(1994),Industrial Mens Cotton Reflective Tape Work Pants.The Journal of the American Leather Chemists Association (JALCA)89(7)[TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye. Analytical Biochemistry212,436-445[Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Coil with AA3003 AA3105 H14 H16 H18. Biotechnology letters 14,6, 531-534[3mm White Waterproof PVC Foam Board]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel 304 Wire Rope Fork Terminal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black and White EVA Sheet for Automotive Packaging]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[15K High Frequency Online UPS UPS RS232/USB/Snmp Optional]

Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Braided PTFE Hose R14 Standard Pipe]

Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck]

Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Power Tools Lithium Battery Cordless Drill (GBK2-66108TS), Part I.CAS 85-44 9 Flakes Phthali Anhydride. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Daytai 1550nm External Modulation Optical Transmitter 1550 -50km]

Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator.High Quality Good Design Custom Made Cheap Recycle Paper Bags, 175-184[Brown Plastic Hangers Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Whitaker,J.R.(1963),Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White. Analytical Chemistry35(12), 1950-1953[Engine Parts Suspension Control Arm OEM 54501-Jg00b]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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