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ABSTRACT

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Key words;Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module;Glass Water Jet Cutting Machine (Skwj-4018A); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.U. S. Type Clevis Grab Hook, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, 1996;Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Car Trunk Organizer Car Storage Organizer,1991).Fashion Cotton Baby/Kid Hat (DH-BF332). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line,OEM Round Stainless Steel Fruit Plate Tray,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Supply Industry Grade Sodium Gluconate(Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, 1996). The soluble protein may have commercial use as gelatines,Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, peptides or amino acids production (En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, 1991;Mini High Temperature Clothing Labels Screen Printing Machine Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Conventional Best-Selling Household Bamboo Craft Chopsticks.High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce Marked Medical Syringe Pump (WP08N)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Geotextile for Roofs, Civil Applications. Railway Work, Parana State,Glass Disc Suspension Insulators.Pneumatic Shuttle Valve Flange Ends, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChinese Color Steel Glazed Tile Purlin Roll Forming Machineand150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror.

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Stx825 Dual 15 Powerful PA Speaker, Speaker Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest).Ce Approval Biscuit Trayless Packing Machine (MZ-250B)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes⁰C for 24 h. The sample was then treated with 1%Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible⁰C for 2 h. After this treatment,Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter, as shown inPractical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts.

Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm⁰C and left for 2 h (Ballscrew Fixed Support Unit Fk12 with High Quality for The Module). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Pattern Acrylic Sheet for Decoration Produced by Senpu. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16. Proteins were also determined by Biuret and Kjeldhal'SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends(Rectangular Lunch Tin Box with Handle). The filtrate in branch B was treated in the same way as branch B inwww.fashiongusta.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSupply 20-110mm PP Reducing Tee for Pipe Systems(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solid Carbide Single Flute End Mills Oblique Angle Cutting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabweshows the peptides separations after enzymatic hydrolysis.Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWeak Daylight Recharge All in One Solar Street Light, but not shown in SBPP,2019 New Release Doypack Sachet Powder Packing Machine Price, and also free amino acids (Whitaker et al., 1963).Amplifier Tube Glass Vacuum Tube (833C). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale,1996).

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CONCLUSIONS

Hot Promotion Indian Market Underwear Adhesive Glue. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera) for the financial support.

REFERENCES

700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2017 Best Price 15W Integrated LED Solar LED Road&Street Light]

Brown, E. M; Thompson, C .J.,Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files, M.M.(1994),New Industrial Wheeled Wooden Shoe Display Fixtures for Shop.The Journal of the American Leather Chemists Association (JALCA)89(7)[Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Injection Molding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness. Analytical Biochemistry212,436-445[12/24V 150ah Power Battery Charger]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment. Biotechnology letters 14,6, 531-534[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Heidemann, E.(1997),Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light.Leder 48,(5),99-104[D-Alpha Tocopherols 1000iu]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Women Summer Swimsuit Chest Zip Bathing Suit]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Finely Processed Pneumatic Operated Globe Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Precision Green Sand Casting Ball Valve Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Palm Oil Processing Press Milling Machine Palm Oil Extraction Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Spped Weaving Machine 6]

China Spplier 70W Solar Street Light with Camera Monitoring System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Car Parking or Access Control 2.4 GHz Active RFID Card]

China Spplier 70W Solar Street Light with Camera Monitoring System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

China Spplier 70W Solar Street Light with Camera Monitoring System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard, Part I.Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush.Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory, 175-184[Cold Room Door Rubber Seal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

Whitaker,J.R.(1963),Masturbators Adult Sex Toys Japanese Love Doll. Analytical Chemistry35(12), 1950-1953[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion LineAuthor for correspondence