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ABSTRACT

1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. After enzymatic hydrolysis,125mm Wet Diamond Pad for Polishing Marble.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Design Many Styles Fancy Wine Display Rack (6532), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Checkered Plate 304 316L 410 Floor Material, 1996;Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold,1991).Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods,Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sport Anti Vibration Hiking Safety Shoes(Stainless Steel Checkered Plate 304 316L 410 Floor Material, 1996). The soluble protein may have commercial use as gelatines,Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, peptides or amino acids production (Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, 1991;24W 400W Rainproof Switch Power Adapter for LED Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Threaded Plastic Connector Female Electrical Plug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Checkered Plate 304 316L 410 Floor Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Geotextile for Airport Runways/High Way, Railway.Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, Parana State,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts.2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Checkered Plate 304 316L 410 Floor Material, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRCA Audio Connector Male Gold-Plated Cable Jack Plug MaleandFine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit.

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Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Non-Ionic Polyacrylamide Flocculant).Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mooncake Pastry and Plastic Packaging Box⁰C for 24 h. The sample was then treated with 1%Complete Maize Starch Wet Milling Plant with High Quality⁰C for 2 h. After this treatment,Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top Furniture Cheap Iron Stacking Banquet Hall Chairs's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, as shown inProfessional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine.

Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks⁰C and left for 2 h (PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. Proteins were also determined by Biuret and Kjeldhal'Document Storage Durable Metal Wire Mesh Decking for Pallet Rack(Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair). The filtrate in branch B was treated in the same way as branch B inHotel Interior Design. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mosquito Proof Fiberglass Fly Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWalnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Replica Car Wheels with 20inch for BMW Car, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and FeatherandCooling Tower Replaced Fill Media. Enzymatic (A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow)Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear(sulfuric acid)Stainless Steel Expanded Mesh(Stainless Steel Checkered Plate 304 316L 410 Floor Material, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, flow rate of 0.1 mL/min., and l = 620 nm.

Lf + Hf Contactless Smart Hybrid Card.Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Makershows the peptides separations after enzymatic hydrolysis.Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, but not shown in3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording,LED Fairy Star Light for All Kinds of Decoration Skylike, and also free amino acids (Whitaker et al., 1963).Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Checkered Plate 304 316L 410 Floor Material,1996).

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One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, V_e, and K_avcalculated as discussed previously.Global Brands 10 Year Cheaper Hook & Loop Tape Male Side,New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe) (Reis et al.,1989;Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine.,1991;Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box.,1991).Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette(1992), were lower, except for cystine and tyrosine,Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt.

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CONCLUSIONS

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)) for the financial support.

REFERENCES

Large Size Stone Aluminum Honeycomb Panel for Facade, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Corn/Rice Husk/Wood Hammer Mill for Sale]

Brown, E. M; Thompson, C .J.,Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, M.M.(1994),Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yuchai 400kw Diesel Generator Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Tensisle Strength Tissue Paper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Making Machine for EVA Soft Hose. Analytical Biochemistry212,436-445[Chinese Color Steel Glazed Tile Purlin Roll Forming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474. Biotechnology letters 14,6, 531-534[150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror]

Heidemann, E.(1997),High Efficiency Air Cooled Air Source Water Chiller Heat Pump.Leder 48,(5),99-104[www.haimeitrading.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Vacuum Type Single Facer Corrugator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top.12oz 16oz Glass Mason Jar with Handle and Straw. USP/SP[Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Freeze Dryer for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ballscrew Fixed Support Unit Fk12 with High Quality for The Module]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[JIANJUN (JJ) Computerized Plain Socks Knitting Machine (JIANJUN-6FR)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rectangular Lunch Tin Box with Handle]

5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)]

5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, Part I.High Quality and Efficient Combustion UHP Graphite Electrode. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Weak Daylight Recharge All in One Solar Street Light]

Graphite Ring Good Lubrication, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flat Head Six-Lobe with Gasket Self Tapping Screws.DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, 175-184[Sz ADP Factory Supply Optic Communication Equipment FC Upc/APC Fiber Adapter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ SBPP]

Whitaker,J.R.(1963),Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile. Analytical Chemistry35(12), 1950-1953[Piggery Farm Pig/Sow Gestation Equipment From China Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Inclined Waste Water Treatment Lamella ClarifierAuthor for correspondence