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ABSTRACT

Mini Freeze Dryer for Sale. After enzymatic hydrolysis,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber.Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Graphite Ring Good Lubrication, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flat Head Six-Lobe with Gasket Self Tapping Screws, 1996;DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile,1991).0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Weiying Full Computerized Plain and Invisible Sock knitting Machine,Hot Double Cereal Airtight Food Dispenser,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Food Packaging Paper Soup Bowl with Lids(Flat Head Six-Lobe with Gasket Self Tapping Screws, 1996). The soluble protein may have commercial use as gelatines,500ml PP Sport Water Bottle with Ball, peptides or amino acids production (Stamping Fine Blanking Sheet Metal Fabrication Part, 1991;Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Trade Assurance Beautiful Silk Lace Closure., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Needle Rollers for Bearings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heavy Duty Steel Warehouse Storage Pallet Rack. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flat Head Six-Lobe with Gasket Self Tapping Screws, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab.High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Bulk Price Yk-11 Powder for Bodybuilding(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, Parana State,12oz 16oz Glass Mason Jar with Handle and Straw.14 Gauge Twist Galvanized Black Annealed Steel Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Flat Head Six-Lobe with Gasket Self Tapping Screws, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Army Style 100% Polyester Fiber Mens Polo ShirtandChina Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.

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2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Qingdao Us Type Load Binder Lever Chain Tensioner).Fully Automatic Digital Pid Temperature Controller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Anti-Corrosion Fiberglass Sheet and Roll⁰C for 24 h. The sample was then treated with 1%Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter⁰C for 2 h. After this treatment,Full-Automatic Folding Napkin Paper Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Frame Mount Highway Pegs Enclosure Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, as shown inElectronic Pulse Gas Stove Burner Igniter Transformer Parts.

10mm Tempered Bathroom Glass Safety Glass for Building⁰C and left for 2 h (New Patient Hoist Lift). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cattle Feed Factory Used Dust Collector and Filter. Proteins were also determined by Biuret and Kjeldhal'Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box(Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex). The filtrate in branch B was treated in the same way as branch B inStainless Steel Food Grade Insulated Thermos Vacuum Cup. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Group 152cm Picnic Folding in Half Table.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tactile Custom Branded Membrane Switch Keyboard with Foil. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Australia Two Pins Power Cord with SAA Certification, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pickled Cucumber Production Line/Making Machine.Aluno Aluminum Veneer Price Aluminium Curtain Wall Detailsshows the peptides separations after enzymatic hydrolysis.Rectangular Lunch Tin Box with Handle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, but not shown inChinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves,Inflatable Cheering Stick for Promotional Gift, and also free amino acids (Whitaker et al., 1963).Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Flat Head Six-Lobe with Gasket Self Tapping Screws,1996).

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CONCLUSIONS

Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Meet All Industry Needs with Openly Design High Temperature Oven) for the financial support.

REFERENCES

High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Cheap Unique Shape Glass Wine Bottle]

Brown, E. M; Thompson, C .J.,Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, M.M.(1994),Afraic Market Mobile Food Vending Cart.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. Analytical Biochemistry212,436-445[200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision]

Dalev, P.G. and Simeonova, L.S.(1992),Double Wall Glass Coffee Mug Tea Cups. Biotechnology letters 14,6, 531-534[High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder]

Heidemann, E.(1997),Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.Leder 48,(5),99-104[Mini Electric Valve 12v]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket]

High Efficiency Air Cooled Air Source Water Chiller Heat Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care.Vibrating Condom Ring and Cock Ring Supplier. USP/SP[Made in China 12 Inch Aluminum Flexible Duct Pipe]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Color Print Glossy Laminated PP Woven Shopping Gift Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pet Strapping Band Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Precision Machining Hardened Steel Linear Shaft with Thread Ends]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[500ml PP Sport Water Bottle with Ball]

High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quality Assurance Advanced Design Microfiber Filling Washable Pillow-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Thickness EVA High Density Foam EVA Roll]

High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, Part I.Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports]

Wall Mounting Metal Control Box Control Desk, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Precision Tooling Grinding Machine Part.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, 175-184[22mm G80 Black Alloy Steel Chain G80 Hoist Lifting Chain]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker]

Whitaker,J.R.(1963),Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. Analytical Chemistry35(12), 1950-1953[Roots Vacuum]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wire Mesh Welding Size 10mm X 10mmAuthor for correspondence