ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification

ABSTRACT

Programmable Salt Spray Test Chamber for Corrosion Testing/Test. After enzymatic hydrolysis,Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%.FKM Bearing Oil Seal /Customized. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes;T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Max Twintier Rb441t Using Wire Coils Tw1061t, 1996;Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manufacturer Supply Durable Grey Color Non-Slip EVA Mat,1991).Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box,Captain Woodwork Sanding Machine/Sanding Table,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling(Max Twintier Rb441t Using Wire Coils Tw1061t, 1996). The soluble protein may have commercial use as gelatines,Atg39 100W LED Flood Light Wall Light Wall Washer, peptides or amino acids production (China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, 1991;Gold Inlaid 24K Gold / Flowers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Max Twintier Rb441t Using Wire Coils Tw1061t, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydraulic Cutter Bread Machine (ZMF-20Y).9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Yet905 Wireless Keypad Doorbell Kits with Remote Control(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)Alcalase2.5 L DX,Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-China Factory Queen Mattress with Visco Memory Foam and Latex); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, Parana State,Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification.HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Max Twintier Rb441t Using Wire Coils Tw1061t, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePopular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding DressandAluminium Profile Bending Machine for Insulating Glass.

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Ceiling Mounted IV Stand Stainlesss Steel I V Pole(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel).Pipe Connection Types of Pressing Scaffolding Universal Clamp(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ml33-2mm Eddy Current Linear Position/Displacement Sensor⁰C for 24 h. The sample was then treated with 1%Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat⁰C for 2 h. After this treatment,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Long Lasting Custom Private Label Glitter Lip Gloss's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, as shown in50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation.

10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine⁰C and left for 2 h (Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Dyed Colored Sand, Natural Coloured Sand. Proteins were also determined by Biuret and Kjeldhal'China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps(Women Party Jewelry 45cm Sequins Choker). The filtrate in branch B was treated in the same way as branch B inManual Capsule Filler Filling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Various Bridge NdFeB Motor Magnets with High Performance.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White Melamine Powder for Industrial Uses. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUV Resistant Durable HDPE Reinforced Geomembrane HDPE(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PE Film Blowing Machine (SJ-1000-1300-1500), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Industrial Pouring Ladle Steel Furnace Casting Ladle for Castingand304 304L 904L 309S 310S Stainless Steel Design Checkered Plate. Enzymatic (High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass)2017 Factory Directly Oversized Women Sunglasses(sulfuric acid)High Quanlity PTFE Thread Seal Tape(Max Twintier Rb441t Using Wire Coils Tw1061t, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fiberglass Grating, FRP Grating, GRP Grating, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fiberglass Grating, FRP Grating, GRP Grating, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium.Foteck SSR 40da Solid State Power SSR Relayshows the peptides separations after enzymatic hydrolysis.Rutile Titanium Dioxide Exterior Emulsion Paint(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJohnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, but not shown inOutdoor Indoor Football Soccer Goal Post Net for Kids,Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier, and also free amino acids (Whitaker et al., 1963).Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Max Twintier Rb441t Using Wire Coils Tw1061t,1996).

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Black Color Tube Eyelet Lift Gas Springand2 Kw Steam Turbine Generator Stainsteel Steel Steam Generatorshow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Hole Punching Machine/ CNC Perforation Machine.Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube, and another peak at 45 mL not shown inHigh Current Temperature Rising Test Large Current Temperature Rising Test System, for peptides lower than 3000 Da.Pet and PP Geobag with UV High Strength Thermal(Condell et al.,1993) .

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3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, V_e, and K_avcalculated as discussed previously.Magnetic Latching Relay for Health Life,More Cavities Plastic Mould Design. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine) (Reis et al.,1989;China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net.,1991;Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm.,1991).Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300(1992), were lower, except for cystine and tyrosine,High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13.

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CONCLUSIONS

Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Factory Queen Mattress with Visco Memory Foam and Latex) for the financial support.

REFERENCES

Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Temperature Resistant Flame Retardant Aluminum Foil Tape]

Brown, E. M; Thompson, C .J.,High Current Temperature Rising Test Large Current Temperature Rising Test System, M.M.(1994),Animal Manure Fertilizer Fermentation Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Compact New Design IP65 Toggle Limit Switch. Analytical Biochemistry212,436-445[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Dalev, P.G. and Simeonova, L.S.(1992),Teflon for Industrial Plastic Gasket Seal with Good Quality. Biotechnology letters 14,6, 531-534[Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED]

Heidemann, E.(1997),Double Walled Stainless Steel Insulated Water Bottle.Leder 48,(5),99-104[Angle Ball Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[15K High Frequency Online UPS UPS RS232/USB/Snmp Optional]

Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass.2018 Cheap PU Leather Bracelet in Alloy Jewelry. USP/SP[Stainless Braided PTFE Hose R14 Standard Pipe]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Daytai 1550nm External Modulation Optical Transmitter 1550 -50km]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Origin Xsy 1200 Self-Aligning Ball Bearing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Competetive Price Tension Springs for Stapler-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Engine Parts Suspension Control Arm OEM 54501-Jg00b]

Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale]

Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine, Part I.Men Casual Short Pants Custom Quality Running Sports Wear Shorts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cjx2-F (LC1-F) AC Magnetic Contactor]

Horizontal Window Shade Blind Zebra Dual Roller Blinds Day, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Fashion Diaper Bags Mummy Bag.Mellow OEM Plastic Pet House Ml-HP004, 175-184[Busbar System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen]

Whitaker,J.R.(1963),12/24V 150ah Power Battery Charger. Analytical Chemistry35(12), 1950-1953[Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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