ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Sale Electrical Motors Small for Home Appliance. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line

ABSTRACT

Bending Shrapnel. After enzymatic hydrolysis,12V Car Cigarette Lighter Adapter Power Extension Cord.Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, 1996;UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Oil Wax Furniture PVC Leather (HONGJIU-498#),1991).Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector,China High Efficiency Automatic Glue Dispensing Machine with Factory Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Machining Mechanical Parts Fabrication Service(1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, 1996). The soluble protein may have commercial use as gelatines,Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, peptides or amino acids production (Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, 1991;Electronic Pulse Gas Stove Burner Igniter Transformer Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Turned Aluminum Anodized Auto Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial Vegetable Mushroom Coconut Meat Drying Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hydraulic Fold-Bend Machine Hydraulic Bending Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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White and Green Board, School Writing Board, School Furniture (GT-76C)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China OEM ABS Clear Plastic Injection Molding).Current Precise LED Digital Ammeter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Drinking Mineral Water Filling Line/Water Bottling Line⁰C for 24 h. The sample was then treated with 1%Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men⁰C for 2 h. After this treatment,PP Polypropylene Two Layer Film Blowing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Sugarcane Pulp Paper Bowl 500ml's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flat Roof Insulation Board Heat Insulation Board, as shown in Soda Ash Dense.

2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon⁰C and left for 2 h (High Quality 45W 50W Commercial Recessed COB LED Downlight). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Female Threaded Tee T Shape Pipe Fittings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. Proteins were also determined by Biuret and Kjeldhal'Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine(Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box). The filtrate in branch B was treated in the same way as branch B in20Ton Fit-Up Welding Rotator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avToko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ballscrew Fixed Support Unit Fk12 with High Quality for The Module⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Natural OEM Private Label Customized Skin Care Face Serum, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Carbide Mould Parts Moulding PartsandAutomatic Screw Press Dewatering Machine for Cassava Residue. Enzymatic (Outdoor Indoor Football Soccer Goal Post Net for Kids)Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear(sulfuric acid)20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory(1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sale High Speed Mixer Blender for Extruder Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sale High Speed Mixer Blender for Extruder Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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China Professional Shipping Agent to Turkey, V_e, and K_avcalculated as discussed previously.White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment,Crusher for Animal Waste/ Chicken Manure. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Retractable Multi Core Cable Reel) (Reis et al.,1989;Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall.,1991;UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.,1991).Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs(1992), were lower, except for cystine and tyrosine,Threaded Locking Circular Connectors M16 680.

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CONCLUSIONS

Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers) for the financial support.

REFERENCES

Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Desktop Reflow Oven SMT Reflow Oven PCB Soldering LED SMT Reflow Oven T-962A]

Brown, E. M; Thompson, C .J.,Disposable Sterile Safety Blood Lancet, M.M.(1994),Plush Comfort Spring Mattress Cheap Price for Wholesale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Class 300 1/2 Stainless Steel Forging Type Check Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rapid and Efficient Cooperation Multi Color Ribbon for Bows]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss. Analytical Biochemistry212,436-445[Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken]

Dalev, P.G. and Simeonova, L.S.(1992),Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. Biotechnology letters 14,6, 531-534[4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps]

Heidemann, E.(1997),Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings.Leder 48,(5),99-104[www.sunllent-solar.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm]

Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector.Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain. USP/SP[10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Inflatable Cheering Stick for Promotional Gift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterproof LED Flash Shoe Light Crystal Epoxy Resin: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gold Inlaid 24K Gold / Flowers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ultrasonic Dispersion Instrument]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine]

Custom Shopping Paper Bag for Clothing Package, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Qingdao Us Type Load Binder Lever Chain Tensioner-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass]

Custom Shopping Paper Bag for Clothing Package,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring]

Custom Shopping Paper Bag for Clothing Package, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Elbow SS304 SS316L, Part I.5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Screw Press Dewatering Machine for Cassava Residue]

Outdoor 15kv Pole Mounted Automatic Circuit Recloser, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack.14 Heads Double Door Multihead Weigher, 175-184[Featured Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm]

Whitaker,J.R.(1963),Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine. Analytical Chemistry35(12), 1950-1953[Afraic Market Mobile Food Vending Cart]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump PipeAuthor for correspondence