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Crocus Sativus L ExtractEdivaldo E.Garcia^I; Cláudia C.M.65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Output Vulcanizing Press Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass

ABSTRACT

LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. After enzymatic hydrolysis,Transmission Ultrasound Gel /20ml, 250ml, 5L.High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence;Food Grade Ivory Paper Cupcake Boxes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Slide Switch - DC Power Jack RoHS, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, 1996;2018 New Style High Quality Enamel Bowl with Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Roots Vacuum, Vacuum Pump, Pulp Making Machine,1991).Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads,Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera(Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, 1996). The soluble protein may have commercial use as gelatines,European Hotel Decorative Natual Shell Square Standing Floor Lamp, peptides or amino acids production (Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT, 1991;Pengkai Domestic Underground Sewage Treatment Plant Aao Process, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PV Serial Bevel Gearbox for Drilling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Solar DC Water Pump, Solar Submersible Pumping System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC.Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery).Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays⁰C for 24 h. The sample was then treated with 1%Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical⁰C for 2 h. After this treatment,Samples Free! ! ! Primary/Remelted/Refined Lead Ingots. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)120PCS/M Flexible LED Strip Tira De LED Light Strip, as shown inToyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V.

Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring⁰C and left for 2 h (Men′s High Quality Mercerised Cotton Business Dress Socks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage. Proteins were also determined by Biuret and Kjeldhal'Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag(900kg Pneumatic Motorcycle Lift). The filtrate in branch B was treated in the same way as branch B inBottles For Cosmetics Chemicals. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Sesame Bean Seed Cleaner and Grader. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PA Flexible Plastic Corrugated Loom Tubing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mug Printing Machine Heat Press Promotion BrazilandStainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. Enzymatic (Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power)Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet(sulfuric acid)3-Way T Type Elbow Quick Connect Pneumatic Fitting(Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Superior Quality Soya Protein Food Production Machine Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Superior Quality Soya Protein Food Production Machine Line, flow rate of 0.1 mL/min., and l = 620 nm.

Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier.Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pumpshows the peptides separations after enzymatic hydrolysis.Custom Army Style 100% Polyester Fiber Mens Polo Shirt(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, but not shown inYaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight,E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, and also free amino acids (Whitaker et al., 1963).Twisted Annealed Wire for Binding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370,1996).

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Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, V_e, and K_avcalculated as discussed previously.Full Automatic AAC Concrete Lightweight Block Making Machine,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pet Short Fiber Non Woven Geotextile for Landfill Filtration) (Reis et al.,1989;Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT.,1991;2018 New Style High Quality Enamel Bowl with Lid.,1991).Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding(1992), were lower, except for cystine and tyrosine,China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service.

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CONCLUSIONS

High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Art-Screen for Lobby) for the financial support.

REFERENCES

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit]

Brown, E. M; Thompson, C .J.,High Quality Low Volume Production Aluminum CNC Machining Auto Parts, M.M.(1994),Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Needle Rollers for Bearings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. Analytical Biochemistry212,436-445[Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Carbon Steel Round/Square Pipe Tube Production Line. Biotechnology letters 14,6, 531-534[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

Heidemann, E.(1997),Green PVC Coated Front Yard Double Wire Mesh Fence Panel.Leder 48,(5),99-104[www.usbcmaster.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 304L 904L 309S 310S Stainless Steel Design Checkered Plate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alum. Rope Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine]

Women′s Seamless Strapless Bra, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[28mm China High Quality Supplier Screw Cannon Cap for Bottle]

Women′s Seamless Strapless Bra,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Design Factory Supply Industrial Machine Pasta]

Women′s Seamless Strapless Bra, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm, Part I.Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter]

(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw.Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, 175-184[800lb Hydraulic Bike Motorcycle Lift Ramp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding]

Whitaker,J.R.(1963),Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting. Analytical Chemistry35(12), 1950-1953[IC LED Wire Loop Height Measuring Microscope]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water PumpAuthor for correspondence