ARTICLES

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ABSTRACT

Good Quality Banded Type Tee. After enzymatic hydrolysis,Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FKM Rubber Gasket/Rubber O Rings in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Inclined Waste Water Treatment Lamella Clarifier, 1996;33kv 100kVA Dry-Type Distribution Transformer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale,1991).Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc (Inclined Waste Water Treatment Lamella Clarifier, 1996). The soluble protein may have commercial use as gelatines,China Manufacturer Export Copper Foot Tap/Valve (HFV-001), peptides or amino acids production (Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, 1991;PVC Corrugated or Smooth Surface Helix Suction Water Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ivd Component PP Barbed Threaded Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Workshop Tools 7.5 Ton Electric Winch with Dual Speed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Inclined Waste Water Treatment Lamella Clarifier, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment.Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Efficient Online Hot Glue Dispensing Machine for Electronic Products(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets).High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship⁰C for 24 h. The sample was then treated with 1%Automotive Sheet Metal Stamping Car Auto Parts⁰C for 2 h. After this treatment,Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1, as shown inShenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB.

Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation⁰C and left for 2 h (Carbon Steel Round/Square Pipe Tube Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Patient Hoist Lift. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23). Proteins were also determined by Biuret and Kjeldhal'Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine(1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector). The filtrate in branch B was treated in the same way as branch B in720p CCTV Car/Bus/Truck/RV IR Dome Vehicle Ahd Camera. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Bucket Segment Bolt 6V0937, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts.FKM Bearing Oil Seal /Customizedshows the peptides separations after enzymatic hydrolysis.OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, but not shown inVacuum Type Single Facer Corrugator,Carbon Steel Pipe ASTM A106 A53, and also free amino acids (Whitaker et al., 1963).Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Inclined Waste Water Treatment Lamella Clarifier,1996).

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CONCLUSIONS

Ycom USB Charger and Data Cable with Aluminum for Type C. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Small Paper Gift Box with Magnet Closure for E-Cigarette) for the financial support.

REFERENCES

Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dh85 Cold Spiral Oil Press Machine]

Brown, E. M; Thompson, C .J.,8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, M.M.(1994),Seashell Special Shape Magnetic Eye Shadow Empty Palette.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage. Analytical Biochemistry212,436-445[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. Biotechnology letters 14,6, 531-534[Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB]

Heidemann, E.(1997),Nice Quality Coloring Printing Children Board Book Printing.Leder 48,(5),99-104[Sc/Upc-Sc/Upc Sinplex Sm Fiber Optic Adapter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable Sterile Safety Blood Lancet]

Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808).Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine. USP/SP[Steel Jacket Steam Insulation Pipeline Production Famouse in China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Water Drop Earring Charming 925 Silver Earrings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tie Down Nylon Back to Back Hook and Loop Straps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2alarms/3alarms Spider Security EAS Alarm Tag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Animal Manure Fertilizer Fermentation Machine]

Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Mini School Backpack for Girls]

Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer]

Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5km City Surveillance Network Infrared Laser PTZ Camera, Part I.300 M Portable Water Well Mining Exploration Core Drilling Rig. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum/Aluminium Extrusion Profile Housing for LED Light]

Foteck SSR 40da Solid State Power SSR Relay, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2015 New Square Folding Ajustable Wall LED Bathroom Mirror.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle, 175-184[MM SMD LED RL]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow]

Whitaker,J.R.(1963),13.8cm Reed Diffuser Clear Glass Bottle for Aroma. Analytical Chemistry35(12), 1950-1953[Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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