ARTICLES

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ABSTRACT

Most Popular Split Pressurized Flat Panel Solar Water Heater. After enzymatic hydrolysis,Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter.Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, 1996;RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles,1991).Cog Mono Round Display LCD Module. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Products Suppliers Camlock Coupling Quick Couplings Female(Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, 1996). The soluble protein may have commercial use as gelatines,Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, peptides or amino acids production (3.2m Vertical Wheelchair Elevator Platform for Sale, 1991;LC Multistage Pump Mechanical Seal (KLLC), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wedding Tent Cooling System Outdoor Event Air-Con Units., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Prime Gi Steel Strip Galvanized Metal Strip Price.Fireproof Aluminum Curtain Wall Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (120PCS/M Flexible LED Strip Tira De LED Light Strip).Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%15K High Frequency Online UPS UPS RS232/USB/Snmp Optional⁰C for 24 h. The sample was then treated with 1%Professtional Suppliers Yutong Golden Dragon Bus Spare Parts⁰C for 2 h. After this treatment,Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)220V/50Hz Stainless Steel Ozone Generator with 5g/H, as shown inGrade Aluminum Fly Fishing Thermometer.

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training⁰C and left for 2 h (600mm Warehouse No Power Roof Fan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Luxury Jewelry White Gold Plated Engagement Diamond Ring. Proteins were also determined by Biuret and Kjeldhal'Free Sample Cheap Price Plastic Black Underwear Hanger for Sale(12 Teeth Green Painting Steel Rake). The filtrate in branch B was treated in the same way as branch B inA4 Sheet Protector Exporter Hot Sale Product. The final soluble protein shown were used for peptides separations using gel permeation chromatography,13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% Cotton Face Mask in Guangzhou. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM High Pressure Machined Part Die Aluminum Casting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Drug Delivery ABS Hospital Medical Treatment Medicine Cart, pH 3.25., mobile phase B: buffer solution of 0.01 mol/YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

HOWO 420HP Rhd Tractor Truck HeadandBasalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. Enzymatic (Colorful Electric English Funny Music Story Module Printing Book)2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(sulfuric acid)IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)(Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tilt-and-Turn-with-Hidden-Hinge, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tilt-and-Turn-with-Hidden-Hinge, flow rate of 0.1 mL/min., and l = 620 nm.

Automatic Soft PVC Granule Used for Refrigerator Door Gasket.Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinetshows the peptides separations after enzymatic hydrolysis.0.45um Dia 47mm PVDF Filter Membrane(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Style Custom Logo Makeup Multi Colored Eyeshadow Palette, but not shown in Stc-001/Stc-100A/Stc/8080A+,Commecial Use Watch Display Cabinet for Watch Store Interior Design, and also free amino acids (Whitaker et al., 1963).Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947,1996).

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CONCLUSIONS

High Quality Washable Durable Hotel Polyester Banquet Table Cloths. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Adjustable Grooming Table) for the financial support.

REFERENCES

16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Frozen Oyster]

Brown, E. M; Thompson, C .J.,Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, M.M.(1994),200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. Analytical Biochemistry212,436-445[Feed Storage OEM Grain Storage Silo 1cbm to 200cbm]

Dalev, P.G. and Simeonova, L.S.(1992),Aerobic Fermentation Organic Fertilizer Agitator. Biotechnology letters 14,6, 531-534[Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush]

Heidemann, E.(1997),Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment.Leder 48,(5),99-104[12/24V High Torque DC Self-Balanced Motor for Electric Vehicle/Spin Hard Disc Drives]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Experienced and Professional Plastic Helmet Mold Manufacturer]

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CS1363 a Standard UK 3 Pin Power Plug.Insulation Anti Earthquake Exterior Wall Foam Board. USP/SP[Petrochemical Loading Platform Folding Stairs]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[IP65 Outdoor Waterproof Transparent LED Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth]

Easy Soak off OEM 10ml Gel Nail Polish, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mooncake Pastry and Plastic Packaging Box]

Easy Soak off OEM 10ml Gel Nail Polish,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Safe Delivery Effective Sarms Mk 677 for Bodybuilding]

Easy Soak off OEM 10ml Gel Nail Polish, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Recyclable PU Leather Jewelry Pouch, Part I.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

Activated Alumina Used for Catalyst Carrier in Chemical Industry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 45W 50W Commercial Recessed COB LED Downlight.Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, 175-184[Polished Stainless Steel Pipes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Whitaker,J.R.(1963),1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). Analytical Chemistry35(12), 1950-1953[High Density Insulation Rock Wool Board for Wall]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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