ARTICLES

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ABSTRACT

Nail Stamping Plates Custom Metal Part Sheet Metal Stamping. After enzymatic hydrolysis,Hot Sale Sports Jacket Men Outdoor Waterproof Jackets.Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box;22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Steel Silo for Sale/Steel Hopper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1996;Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Smart Big Hydroponic Indoor Home Garden Planter,1991).China Quality Heavy Duty Globe Control Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack,China Suppliers Laser Marking Equipment of Fiber Laser,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bonfiglioli Ta Series Helical Gearing Gearbox(Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1996). The soluble protein may have commercial use as gelatines,Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, peptides or amino acids production (LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, 1991;Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Large Stock Aerators for Shrimp Farms and Fish Pond., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle..

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Diameter Aluminum Pipe 3004. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory.OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Integrated Waterproof PVC Lamination Bathroom Wall Panels(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Embossed PVC Rexine Car Seat Cover LeatherAlcalase2.5 L DX,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Solar Aluminum Rail Splice Connector for Rail Extention); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Grade Aluminum Fly Fishing Thermometer(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, Parana State,LSR Injection Mold Tooling for Medical Nipple.Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Current Temperature Rising Test Large Current Temperature Rising Test SystemandTBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass.

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Sc Fiber Optic Fast Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger).Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10mm Cubes N52 NdFeB Block Magnets for Electronic Components⁰C for 24 h. The sample was then treated with 1%Watermark Approval Brass Press Equal Fitting Male Tee⁰C for 2 h. After this treatment,China Modern Steel Structure Layer Chicken Farm House Designs. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Forged 316 Stainless Steel Marine Twisted Long D Shackle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, as shown in3mm White Waterproof PVC Foam Board.

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels⁰C and left for 2 h (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. Proteins were also determined by Biuret and Kjeldhal'Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight(Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling). The filtrate in branch B was treated in the same way as branch B in20 L American Type Military Portable Mini Stainless Steel Jerry Can. The final soluble protein shown were used for peptides separations using gel permeation chromatography,355nm UV Laser Marking Equipment for Crystal Materials.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWire Mesh Welding Size 10mm X 10mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plug-in Assembly Type Straight Through Joint and Insulation Joint, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/ShipandHigh Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. Enzymatic (Quilting Machine for Muti- Needle Mattress Quilting Machine)Single-Screw Plastic Extruders Machine(sulfuric acid)Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube(Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quanlity Colorful Aluminum Spacer/Standoff, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quanlity Colorful Aluminum Spacer/Standoff, flow rate of 0.1 mL/min., and l = 620 nm.

5g OPP Silica Gel Desiccant.Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LEDshows the peptides separations after enzymatic hydrolysis.Electric Winch With Groove Drum(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, but not shown inStainless Braided PTFE Hose R14 Standard Pipe,Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, and also free amino acids (Whitaker et al., 1963).Truck Air Dryer cartridge 14bar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality,1996).

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Daytai 1550nm External Modulation Optical Transmitter 1550 -50kmandHigh Quality Industrial Grade Anhydrous Sodium Sulfateshow the gel chromatographic separation of soluble protein with Sephadex G-Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors.3mm White Waterproof PVC Foam Board, and another peak at 45 mL not shown inEngine Parts Suspension Control Arm OEM 54501-Jg00b, for peptides lower than 3000 Da.Educational Equipment Smart Multi Touch Interactive White Board for School(Condell et al.,1993) .

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Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, V_e, and K_avcalculated as discussed previously.Women Party Jewelry 45cm Sequins Choker,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cjx2-F (LC1-F) AC Magnetic Contactor) (Reis et al.,1989;LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L.,1991;Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights.,1991).High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe(1992), were lower, except for cystine and tyrosine,High Freqency PCB with Blind Via.

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CONCLUSIONS

Strong Aluminum Armrest Reclining Hydraulic Barber Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solar Aluminum Rail Splice Connector for Rail Extention) for the financial support.

REFERENCES

Factory SMD3528 60LEDs/M Flexible LED Strip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Selling Transponder Key Blank for Car Key Blank]

Brown, E. M; Thompson, C .J.,Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, M.M.(1994),Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Speaker Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[24W 400W Rainproof Switch Power Adapter for LED Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick. Analytical Biochemistry212,436-445[Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Comfort Furniture Natural Spring Memory Foam Mattress. Biotechnology letters 14,6, 531-534[450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray]

Heidemann, E.(1997),Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume.Leder 48,(5),99-104[Investment Casting Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Modern Steel Structure Layer Chicken Farm House Designs]

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4.2A Type a Type C Pd QC3.0 USB Charger.Ferrous Sulphate Monohydrate 30%Min Factory Wholesale. USP/SP[China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Portable Scooters Folding Electric Scooters for Adults: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Distillation Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tpdl Belt Press Sludge Equipment with Slaughtering Machine]

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jaguar Jb-390 Automatic PCB Loader and Unloader]

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Construction Scaffolding Steel Galvanized Acrow Prop]

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, Part I.Most Popular Split Pressurized Flat Panel Solar Water Heater. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting]

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine.Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, 175-184[OE Quality Rear Brake Drum 15158975 for Chevrolet/Gmc/Buick]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box]

Whitaker,J.R.(1963),Customized UV Drying Heating Machine for Screen Printer. Analytical Chemistry35(12), 1950-1953[Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Motor Bearing 6014 2RS Deep Groove Ball BearingAuthor for correspondence