ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Sc Fiber Optic Fast Connector. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue

ABSTRACT

10mm Cubes N52 NdFeB Block Magnets for Electronic Components. After enzymatic hydrolysis,Watermark Approval Brass Press Equal Fitting Male Tee.China Modern Steel Structure Layer Chicken Farm House Designs. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, 1996;Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight,1991).355nm UV Laser Marking Equipment for Crystal Materials. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB,Wire Mesh Welding Size 10mm X 10mm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,P4.75 Indoor Single Dual Color Advertising LED Display Billboard(Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, 1996). The soluble protein may have commercial use as gelatines,New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, peptides or amino acids production (Plug-in Assembly Type Straight Through Joint and Insulation Joint, 1991;Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quilting Machine for Muti- Needle Mattress Quilting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Single-Screw Plastic Extruders Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube.High Quanlity Colorful Aluminum Spacer/Standoff-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5g OPP Silica Gel Desiccant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3mm White Waterproof PVC Foam Board, Parana State,Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue.Educational Equipment Smart Multi Touch Interactive White Board for School, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSeasoned Cast Iron South Africa Three Legged Potjie PotandRailway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway.

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Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Design Many Styles Fancy Wine Display Rack (6532)).Women Party Jewelry 45cm Sequins Choker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity⁰C for 24 h. The sample was then treated with 1%High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe⁰C for 2 h. After this treatment,High Freqency PCB with Blind Via. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Strong Aluminum Armrest Reclining Hydraulic Barber Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory SMD3528 60LEDs/M Flexible LED Strip, as shown inPA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4.2A Type a Type C Pd QC3.0 USB Charger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFerrous Sulphate Monohydrate 30%Min Factory Wholesale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide).Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Oldshows the peptides separations after enzymatic hydrolysis.Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, but not shown in2017 New Design Vinyl 3D Stone Wallpaper Factory in China,RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, and also free amino acids (Whitaker et al., 1963).Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile,1996).

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CONCLUSIONS

M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Truck Air Dryer cartridge 14bar) for the financial support.

REFERENCES

Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Linlang Shanghai Premium Quality Libbey Clear Glass Candle Holder Glass Candle Jar With Flat Glass Lid]

Brown, E. M; Thompson, C .J.,Prime Gi Steel Strip Galvanized Metal Strip Price, M.M.(1994),Fireproof Aluminum Curtain Wall Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple. Analytical Biochemistry212,436-445[High Quality Good Design Custom Made Cheap Recycle Paper Bags]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml. Biotechnology letters 14,6, 531-534[240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PE Coated Paper for Salt Sugar Sachets Paper Food Grade: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Shock-Proof Show Performance Lighting Tool Flight Case Table for Stage Lighting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ PVC Butterfly Valve Gear Type]

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks]

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Drive Travel Car Inflatable Bed]

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance, Part I.15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Disposable Clear Take Away Plastic Salad Bowl Forming Machine]

Whitaker,J.R.(1963),220V/50Hz Stainless Steel Ozone Generator with 5g/H. Analytical Chemistry35(12), 1950-1953[3000lb Small Boat Marine Electric Trailer Winch Power-in]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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