ARTICLES

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ABSTRACT

Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). After enzymatic hydrolysis,(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station.off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, 1996;20 Experiences on Plastic Injection Moulding Service., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wind Protection Screen. Wind Dust Netting, Wind Dust Net,1991).24K Gold Rolling Paper Shine Papers Cigarette Paper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Two - Way Double Row Thrust Tapered Roller Bearings,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ride on Toys Riding Animals in The Mall(Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, 1996). The soluble protein may have commercial use as gelatines,Finely Processed Pneumatic Operated Globe Valve, peptides or amino acids production (Color Coated Corrugated Galvanized Roofing Steel Plate, 1991;Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision Diamond Wire Saw Rope for Cutting Concrete Stone.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Blince High Torque OMR Hydro Motor for Injection Moulding Machine.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:TV Tower Antenna Supports(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tractor Tine Tiller Cultivator).Graphic 240X128 Dots Monochrome LCD Display Module(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dry & Wet Concrete Surface Metal Grinding Wing⁰C for 24 h. The sample was then treated with 1%Workbench Anti-Static Table ESD Natural Rubber Table Mat⁰C for 2 h. After this treatment,316L 10 Micron Sintered Stainless Steel Filter Disc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Abstract Light and Shadow Metal Oil Painting for Deco, as shown inZhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.

Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)⁰C and left for 2 h (5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheapest Container Living House/Office/Kiosk. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Spin Centrifugal Demister Separator or Column Internal. Proteins were also determined by Biuret and Kjeldhal'Baby Wet Wipe Machine(Car Trunk Organizer Car Storage Organizer). The filtrate in branch B was treated in the same way as branch B in9060 Laser Engraving And Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av360 Degree Reciprocating Car Wash Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Die Aluminum Castings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Meat Pie Making Machine with Different Mould Shape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gear Operated Flanged High Performance Butterfly Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer CNC High Precision Machining Metal Parts Customized for AutoandChina Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. Enzymatic (Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3)Wholesale Universal Garden Smart Flower Pot(sulfuric acid)Reflective Tape for Vehicle Reflective Markings (tape-02)(Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%White Crystal Powder Betaine Anhydrous CAS#107-43-7, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%White Crystal Powder Betaine Anhydrous CAS#107-43-7, flow rate of 0.1 mL/min., and l = 620 nm.

Creative New Product Coin Holder Keychain.Small Capacity Automatic Cup Filling Machineshows the peptides separations after enzymatic hydrolysis.Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina High Efficiency Automatic Glue Dispensing Machine with Factory Price, but not shown inDiameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning,Weak Daylight Recharge All in One Solar Street Light, and also free amino acids (Whitaker et al., 1963).Ybz16-150/140 Vane Steering Hydraulic Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal,1996).

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Automatic CNC PU Sandal Pouring Machine, V_e, and K_avcalculated as discussed previously.Glass Disc Suspension Insulators,2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home) (Reis et al.,1989;Color Coated Corrugated Galvanized Roofing Steel Plate.,1991;20 Experiences on Plastic Injection Moulding Service.,1991).Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic(1992), were lower, except for cystine and tyrosine,Polypropylene Fibre for Commercial Slabs.

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CONCLUSIONS

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Xcm 120 Asphalt Mixing Plant for Sale) for the financial support.

REFERENCES

Glass Water Jet Cutting Machine (Skwj-4018A), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hotel Bathroom Black Golden Round Metal Framed LED Light Mirror with Defogger]

Brown, E. M; Thompson, C .J.,U. S. Type Clevis Grab Hook, M.M.(1994),Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[304 Precision Professional Dimpled Stainless Steel Plate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Compression Mens Customized Gym Shorts Sportswear]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. Analytical Biochemistry212,436-445[Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng]

Dalev, P.G. and Simeonova, L.S.(1992),Car Trunk Organizer Car Storage Organizer. Biotechnology letters 14,6, 531-534[Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase]

Heidemann, E.(1997),Fashion Cotton Baby/Kid Hat (DH-BF332).Leder 48,(5),99-104[Organic Board-Laser Handicraft Engraving Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Price Promotion Soda Ash Light to Papermaking]

Women′s Seamless Strapless Bra, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.OEM Round Stainless Steel Fruit Plate Tray. USP/SP[Small Bar Fridge with Glass Door for Beverage and Beer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supply Industry Grade Sodium Gluconate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Plastic GRP FRP PE Pipe Fitting for Water Supply]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vhf/Uhf Antenna]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet]

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer]

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X]

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini High Temperature Clothing Labels Screen Printing Machine Factory, Part I.Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lithium Battery 20ah Pouch 3c Cell 3.7V]

Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts.Conventional Best-Selling Household Bamboo Craft Chopsticks, 175-184[www.swinysteel.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Powder Spray Booth for Metal Furniture]

Whitaker,J.R.(1963),High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange. Analytical Chemistry35(12), 1950-1953[High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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